中文摘要：研究人员知识和技能的进步和提高及可用设备的技术革新推动着食品和饲料业测定污染物的分析方案不断发展。霉菌毒素是全球性农业真菌源污染物，依物种的敏感性，对人和动物健康具有急性和慢性影响。食品中的主要霉菌毒素是黄曲霉毒素B1和赭曲霉毒素A，首先是其毒性，其次是其频繁发生。欧盟立法规定了霉菌毒素，如黄曲霉毒素B1、赭曲霉毒素A、脱氧雪腐镰刀菌烯醇、伏马毒素和玉米赤霉烯酮的最大限量，以及T2和HT2毒素的指示性限量。由于确有同一食品或饲料中多种霉菌毒素共现的可能，所以，可靠、灵敏而通用的多霉菌毒素方法的可用性非常重要。由于易受霉菌毒素污染的基质范围广泛及可能的毒素共存，本研究用液相色谱-串联质谱（LC-MS/MS）验证了多霉菌毒素和多基质方法，其目的是克服特定的基质影响，分析意大利总膳食研究计划中的复杂谷物样品。
外文摘要：The analytical scenario for determining contaminants in the food and feed sector is constantly prompted by the progress and improvement of knowledge and expertise of researchers and by the technical innovation of the instrumentation available. Mycotoxins are agricultural contaminants of fungal origin occurring at all latitudes worldwide and being characterized by acute and chronic effects on human health and animal wellness, depending on the species sensitivity. The major mycotoxins of food concern are aflatoxin B1 and ochratoxin A, the first for its toxicity, and the second for its recurrent occurrence. However, the European legislation sets maximum limits for mycotoxins, such as aflatoxin B1, ochratoxin A, deoxynivalenol, fumonisins, and zearalenone, and indicative limits for T-2 and HT-2 toxins. Due to the actual probability that co-occurring mycotoxins are present in a food or feed product, nowadays, the availability of reliable, sensitive, and versatile multi-mycotoxin methods is assuming a relevant importance. Due to the wide range of matrices susceptible to mycotoxin contamination and the possible co-occurrence, a multi-mycotoxin and multi-matrix method was validated in liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the purpose to overcome specific matrix effects and analyze complex cereal-based samples within the Italian Total Diet Study project.
外文关键词：mycotoxin；aflatoxins；ochratoxin A；deoxynivalenol；fumonisins；T-2 and HT-2 toxins；zearalenone；Total Diet Study；liquid chromatography-tandem mass spectrometry
作者：De Santis, B；Debegnach, F；Gregori, E；Russo, S；Marchegiani, F; Moracci, G；Brera, C
作者单位：Ist Super Sanita
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：5
点击下载：开发LC-MS/MS方法，测定复合谷物样品中的多霉菌毒素