中文摘要：本研究开发了一种快速、便携和半定量的免疫层析试纸条，用于快速、可视化检测交链孢酚单甲醚（AME）。为此，制备并鉴定了抗AME单克隆抗体（mAb）。研究表明胶体金免疫层析条可用作半定量工具，现场、快速、可视化检测水果中的AME。
外文摘要：A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1-10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.
外文关键词：alternariol monomethyl ether (AME)；monoclonal antibody；immunochromatographic strip test；colloidal gold nanoparticle；competitive ELISA
作者：Man, Y；Liang, G；Jia, FC；Li, A；Fu, HL；Wang, M；Pan, LG
作者单位：Beijing Acad Agr & Forestry Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：5
点击下载：开发一种免疫层析试纸条用于快速检测水果中的交链孢酚单甲醚