中文摘要：通过使用黄曲霉毒素M1（AFM1）-牛血清白蛋白偶联物、抗AFM1抗体、Eu标记的羊抗兔抗体，开发了用于检测牛奶中AFM1污染的间接竞争性时间分辨荧光免疫测定法（TRFIA）。研究表明，该方法用于筛选大量样品时，简单、灵敏、特异性、可重复性、经济、适用，具有良好的应用前景。
外文摘要：A competitive indirect time-resolved fluoroimmunoassay (TRFIA) for detecting aflatoxin M1(AFM1) contamination in milk was developed, by using aflatoxin M1-bovineserum albumin conjugate, anti-AFM1 antibody, and Eu-labeled goat anti-rabbit antibody. To improve the sensitivity of the assay, the concentrations of the coating antigen and anti-AFM1 antibody were varied to optimize the condition of the immunological assay. The limit of detection values, limit of quantification values, and dynamic working range were 0.006, 0.022, and 0.022–1.334 μg/kg, respectively. Values of recovery within and between assays were 88.0–116.0% and 92.69–108.63%. The method was applicable for the full-cream, semi-skimmed, skimmed, and raw milk as well. Values of repeatability (intra-laboratory variability) and reproducibility (inter-laboratory variability) were 1.2–4.5% and 0.8–5.0%, respectively. The results of using AFM1-TRFIA to analyze samples of 23 brands of milk that were purchased in Wuxi revealed that AFM1 was absent from all studied samples. This study suggests that the novel method is a simple, sensitive, specific, reproducible, economic, and adequate method for screening large quantities of samples and has good prospects of application.
外文关键词：Time-resolved fluoroimmunoassay；AFM1；Europium；Milk
作者：Guo, MM；Zhou, B；Huang, ZJ；Zhao, CC；Zhang, J；Huang, B
作者单位：Jiangsu Inst Nucl Med
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：8
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