中文摘要：本研究使用磁性纳米粒子（MNPs）作标记，开发了高灵敏度免疫色谱测试系统，检测玉米赤霉烯酮（ZEA）和T-2毒素（T2T）。研究结果证实了实际使用磁性纳米粒子作免疫层析测定标记的可行性。
外文摘要：Highly sensitive immunochromatographic test systems were developed for the detection of zearalenone (ZEA) and T-2 toxin (T2T) using magnetite nanoparticles (MNPs) for the labeling. In order to detect an analyte with high sensitivity, the competitive reaction was performed with free specific antibodies, while immune complexes were detected by the reaction with label-conjugated anti-species antibodies. The conditions for the synthesis of magnetite nanoparticles conjugated to anti-species antibodies were optimized. The concentrations of specific reagents that provided the highly sensitive detection of T-2 toxin and zearalenone were found. The instrumental detection limit for the determination of T-2 toxin and zearalenone in baby food samples (oat flakes) was 0.1 and 0.05 ng/mL (2.0 and 1.0 ng/g), respectively. The assay time was 15 min. The results of the present study confirm the possibility of the practical use of magnetite nanoparticles for immunochromatographic assay labeling.
外文关键词：magnetite nanoparticles；immunochromatographic assay；sensitivity enhancement；test strips；zearalenone；T-2 toxin
作者：Petrakova, AV；Urusov, AE；Zherdev, AV；Liu, L；Xu, C；Dzantiev, BB
作者单位：Russian Acad Sci
期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY
期刊影响因子：0.671
出版年份：2017
出版刊次：4
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