中文摘要：局域表面等离子体共振(LSPR)生物传感器允许分子结合中小分子的无标记检测；但受灵敏度低、动态范围窄的限制。本研究报道了利用已知的G-四联体（GQx）粘合剂的GQx结构结合特征提高等离子体传感器的LSPR信号，通过LSPR峰移高灵敏度检测的小分子的方法。研究表明，该等离子体传感器对赭曲霉素A、黄曲霉毒素B1、三磷酸腺苷及钾离子的检测限（LOD）分别为0.56、0.63、0.87及1.05pM，比无黄连素（BER）时高100倍；且动态范围为10pM到10μM。此外，该LSPR传感器还能有效地用于定量分析实际样品中的小分子。
外文摘要：Localized surface plasmon resonance (LSPR) biosensors allow label-free detection of small molecules in molecular binding events; however, they are limited by a relatively low sensitivity and narrow dynamic range. Here, we report highly sensitive small-molecule detection by LSPR peak shift exploiting the G-quadruplex (GQx) structure-binding characteristic of known GQx binders to enhance the LSPR signal of a plasmonic aptasensor. Six known GQx binders (thiazole orange, malachite green, crystal violet, zinc protoporphyrin IX, thioflavin T, and berberine) were tested for their ability to enhance the LSPR signal. Among these, berberine (BER) induced the largest LSPR peak shift by interacting with the GQx structure formed by the aptamer/target binding event on a gold nanorod surface. This specific binding performance was confirmed by the fluorescence signal of BER and through repeated cycles of BER addition and washing on the plasmonic sensing chip. The proposed plasmonic aptasensor respectively showed limit of detection (LOD) of 0.56, 0.63, 0.87 and 1.05 pM for ochratoxin A, aflatoxin B1, adenosine triphosphate and potassium ions, which was 1000-fold higher than that in BER-free condition, and a wide dynamic range from 10 pM to 10μM. In addition, the proposed LSPR aptasensor could effectively be used to quantitatively analyze small molecules in real samples.
外文关键词：Localized surface plasmon resonance (LSPR)；Aptamer；Berberine；Enhancement；Small molecule；Gold nanorod (GNR)
作者：Park, JH；Byun, JY；Jang, H；Hong, D；Kim, MG
作者单位：GIST
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：11
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