中文摘要：Fat-1转基因牛的安全性评估自其出生就成为我们的一个研究焦点。本文综合利用串联质谱标签技术、TiO2富集技术、纳米液相色谱-串联质谱技术来对Fat-1转基因牛和野生牛进行肌蛋白质组学和磷酸化蛋白质组学比较分析。在分析评估中共有1555个肌蛋白质位点和900个磷酸化蛋白质位点被识别，但在Fat-1转基因牛和野生牛间只有4个差异表达的肌蛋白质和9个差异表达的磷酸化蛋白质。生物信息学分析表明，识别到的肌蛋白质和磷酸化蛋白质主要与三大营养素（碳水化合物、脂肪、蛋白质）的代谢过程有关。而差异表达的蛋白质可能与DNA重组与修复、免疫系统调节有关。本文的研究结果位Fat-1转基因牛的安全性评估提供了新的见解。
外文摘要：The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO2 enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows. Bioinformatics analyses showed that all of the identified proteins and phosphoproteins were mainly related to the metabolic processes of three major nutrients: carbohydrates, lipids, and proteins. All of these differentially expressed proteins might take part in DNA recombination, repair, and regulation of the immune system. In conclusion, most of the identified proteins and phosphoproteins exhibited few changes. Our results provide new insights into the biosafety of fat-1 transgenic cattle.
外文关键词：Proteomic; Phosphoproteomic; TMT 6-plex; Fat-1 transgenic cows; Biosafety evaluation
作者：Xin, Xiangbo; Liu, Xinfeng; Li, Xin; 等
作者单位：Tianjin Agr Univ
期刊名称：TRANSGENIC RESEARCH
期刊影响因子：2.341
出版年份：2017
出版刊次：10
点击下载：肌蛋白质组学/磷酸化蛋白质组学比较分析为Fat-1转基因牛的安全性评估提供了新的研究视角