中文摘要：转Bt基因抗虫棉GK 12在国内通常用于鳞翅目害虫的控制，但其对土壤微生物的群落结构可能产生意想不到的效果。本研究利用定量聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）技术，对2009-2011年栽培过转Bt基因抗虫棉GK 12、其近等基因系亲本泗棉3号和常规棉DP 5415的根际土壤中细菌的群落规模和群落结构进行了分析，且测定了脱氢酶、脲酶和磷酸酶的活性。研究发现，这些微生物的相关参数受年份和植物生长阶段的影响显著；在某些生长阶段，细菌群落规模和群落结构偶尔会有差异，但在整个生长周期中没有差异。GK 12和Simian 3棉花的根际土壤酶活性、细菌种群数量和群落结构均无显著差异。GK 12和Simian 3棉花的根际稀土品种不同但不可扩增。研究结果表明，转Bt基因抗虫棉GK 12中Bt基因插入对3年试验的根际土壤酶活性、细菌种群数量和群落结构没有显著且长久的影响。
外文摘要：Transgenic Bt-cotton (Gossypium hirsutum L.) GK 12 is commonly used for control of lepidopteran pests in China, but concerns exist regarding possible unintended effects on soil microbial communities. Bacterial population sizes and community structures in the rhizosphere soil under intensive cultivation of GK 12, its near-isogenic parent Simian 3 and a conventional cotton DP 5415 were analyzed during 2009 to 2011 by quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE). Dehydrogenase, urease, and phosphatase activities were also measured. It was found that these microbial-related parameters were significantly influenced by variations due to the year and plant growth stage. Th ere were occasional differences in bacterial population size and community structures at some growth stages, but they were not manifested throughout the growing cycle and were not considered meaningful. Generally no signifi cant differences were found in selected enzymatic activities, and bacterial population sizes and community structures in the rhizosphere soil of GK 12 and Simian 3. Th ree different species or bands were unique to GK 12 and Simian 3, but they were unamplifiable. The resolvable band profile or annotatable species composition was not different between GK 12 and Simian 3. Therefore, the data of this study did not show any signifi cant and permanent impacts of Bt-insertion in GK 12 on selected enzymatic activities, bacterial population size, and community structures in the rhizosphere soil during 3-yr tests in northern China.
作者：Xie, Ming; Zhang, Yan-jun; Peng, De-Liang; 等
作者单位：Chinese Acad Agr Sci
期刊名称：AGRONOMY JOURNAL
期刊影响因子：1.614
出版年份：2017
出版刊次：8
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