中文摘要：本研究两年间在意大利南部的坎帕尼亚和卡拉布里亚地区随机收集了804份奶牛和水牛鲜奶，评估了它们受黄曲霉毒素M1（AFM1）污染的情况。研究结果表明意大利南部两地牛奶的AFM1污染不构成严重的公共卫生问题。
外文摘要：This study evaluates the aflatoxin M1 (AFM1) contamination in 804 samples of raw milk from cow and buffalo, collected randomly in Campania and Calabria regions of Southern Italy over a two years period. The competitive enzyme linked immunosorbent assay (ELISA) method was used to analyze AFM1 in the samples. AFM1 levels result above the CCβ value of 0.004 μg/kg in 51 (12.3%) cow milk samples and in 28 (7.2%) buffalo milk samples. Positive results from screening analysis were confirmed by high performance liquid chromatography with fluorimetric detection (HPLC-FLD) after a procedure of centrifugation, extraction and immunoaffinity column clean-up of milk. Only one cow milk sample exceeded the maximum limit (0.05 μg/kg) set by the European Regulation. The occurrence of AFM1 contamination was significantly (p < 0.05) higher in cold season, particularly fall, than in warm season, principally spring. Our results indicate that feedstuff used in the buffalo and cow farms were not highly contaminated with aflatoxins, determining a good quality of the analyzed milk. Therefore, the AFM1 contamination of the milk does not represent a serious public health problem in both regions in Southern Italy.
外文关键词：Aflatoxins；Milk；Dairy products；ELISA；HPLC-FLD
作者：De Roma, A；Rossini, C；Ritieni, A；Gallo, P；Esposito, M
作者单位：Ist Zooprofilatt Sperimentale Mezzogiorno
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：11
点击下载：意大利南部水牛和奶牛乳中黄曲霉毒素M1的发生与季节性变化的调查