中文摘要：本文描述了一种基于表面等离子体共振（SPR）的传感器，它以直接测定形式检测致癌真菌毒素黄曲霉毒素B1（AFB1）。它可以测定复杂样品，如稀释的红酒和啤酒中的AFB1。本方法灵敏度高，芯片易于再生且稳定，因此克服了某些用基于抗体的SPR和竞争性SPR测定AFB1的限制。
外文摘要：The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1.
外文关键词：Mycotoxin；SPR；Direct format assay；Affinity binding；Antibody；Chip；Regeneration；Immobilization；Dissociation；Stability
作者：Sun, LL；Wu, LQ；Zhao, Q
作者单位：Chinese Acad Sci
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：8
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