中文摘要：黄曲霉毒素B1(AFB1)是一种毒性最强的化学致癌物，已被广泛研究。开发简单、准确而灵敏的分析方法来检测食品基质中的AFB1仍然具有挑战性。本研究基于双信号策略，设计了一种比率适配体传感器，经验证能准确、灵敏地检测AFB1。该适体传感器表现出良好的选择性、重现性和稳定性，揭示了其在食品安全监测和环境分析中的潜在应用。
外文摘要：Aflatoxin B1 (AFB1), one of the most toxic chemical carcinogens, has been widely studied. It remains challenging to develop simple, accurate and sensitive analytical methods for the detection of AFB1 in food matrices. In this work, based on dual-signaling strategy, a ratiometric aptasensor was designed and verified for the accurate and sensitive detection of AFB1. The electrochemical method was first used as a model to verify the specific interaction between AFB1 and the aptamer, in which ferrocene (Fc) and methylene blue (MB) anchored DNA sequences were acted as dual signals. Consequently, the specific interaction between AFB1 and its aptamer was demonstrated by the "signal-on" mode of Fc and the "signal-off" of MB. Due to the simple dual-signaling mode, the electrochemical sensor was further extended to the construction of electrochemiluminoscence (ECL) aptasensor. In the ECL system, dual ECL signals were produced from CdTe/CdS/ZnS quantum dots (QDs) and luminol. HRP modified gold nanorods (HRP/Au NRs) were acted as the quencher/enhancer which can quench the ECL signal of QDs by ECL energy transfer and simultaneously catalyze H2O2 to enhance the ECL of luminol. Owing to the self-calibration by the internal reference, both of the two ratiometric aptasensors behaved accurate and sensitive analytical performance for AFB1 with a good linear range from 5.0 pM to 10 nM and the detection limit of 0.43 pM and 0.12 pM (S/N = 3), respectively. The aptasensors also exhibited good selectivity, reproducibility and stability, revealing potential applications in food safety monitoring and environmental analysis.
作者：Wu, L；Ding, F；Yin, WM；Ma, J；Wang, BR；Nie, AX；Han, HY
作者单位：Huazhong Agr Univ
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：7.487
出版年份：2017
出版刊次：14
点击下载：从电化学到电致发光：黄曲霉毒素B1比率型传感器的研制与应用