中文摘要：尽管自1997年以来，中国农民种植防治主要棉铃虫的转Bt基因抗虫棉已取得了巨大成功，但很多耐CrylAc蛋白的鳞翅目害虫已对转基因棉花造成了巨大的经济损失。Vip3AcAa蛋白是Vip3Ac N端和Vip3Aa C端的嵌合蛋白，其对鳞翅目害虫具有广谱杀虫活性且与CrylAc蛋白没有交叉抗性。本研究试验了Vip3AcAa蛋白对斜纹夜蛾、甜菜夜蛾和地老虎的杀虫活性。生物测定结果表明，Vip3AcAa蛋白对这三种害虫的杀虫活性要优于CrylAc蛋白；且在活化处理后，Vip3AcAa蛋白对这三种害虫的杀虫活性与未加工的蛋白一致。为了加强转Bt基因抗虫棉的广谱杀虫活性，本研究研发了含Vip3A和Cry1Ac的双价转基因棉花。在实验室实验中，含Vip3A和Cry1Ac的双价转基因棉花对斜纹夜蛾、甜菜夜蛾和地老虎的幼虫死亡率的影响要远远大于常规棉花和转CrylAc基因抗虫棉。研究表明，Vip3AcAa蛋白是中国害虫综合治理策略的一个很好的选择。
外文摘要：Although farmers in China have grown transgenic Bt-CrylAc cotton to resist the major pest Helicoverpa armigera since 1997 with great success, many secondary lepidopteran pests that are tolerant to CrylAc are now reported to cause considerable economic damage. Vip3AcAa, a chimeric protein with the N-terminal part of Vip3Ac and the C-terminal part of Vip3Aa, has a broad insecticidal spectrum against lepidopteran pests and has no cross resistance to CrylAc. In the present study, we tested insecticidal activities of Vip3AcAa against Spodoptera litura, Spodoptera exigua, and Agrotis ipsilon, which are relatively tolerant to CrylAc proteins. The bioassay results showed that insecticidal activities of Vip3AcAa against these three pests are superior to CrylAc, and after an activation pretreatment, Vip3AcAa retained insecticidal activity against S. litura, S. exigua and A. ipsilon that was similar to the unprocessed protein. The putative receptor for this chimeric protein in the brush border membrane vesicle (BBMV) in the three pests was also identified using biotinylated Vip3AcAa toxin. To broaden Bt cotton activity against a wider spectrum of pests, we introduced the vip3AcAa and clylAc genes into cotton. Larval mortality rates for S. litura, A. ipsilon and S. exigua that had fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and Bt-CrylAc cotton in a laboratory experiment. These results suggested that the Vip3AcAa protein is an excellent option for a "pyramid" strategy for integrated pest management in China.
外文关键词：Bacillus thuringiensis; Vegetative insecticidal proteins; Midgut putative receptor; Transgenic cotton; Spodoptera spp
作者：Chen, Wen-bo; Lu, Guo-qing; Cheng, Hong-mei; 等
作者单位：Chinese Acad Agr Sci
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY
期刊影响因子：2.379
出版年份：2017
出版刊次：10
点击下载：含Vip3A和Cry1Ac的双价转基因棉花对鳞翅目害虫具有广谱杀虫活性