中文摘要：本研究首次提出了具有“外部”特异性抗体的免疫层析竞争性分析方法。该方法全球通用，可检测各种各样的化合物。
外文摘要：The scheme of immunochromatographic competitive analysis with "external" specific antibodies is offered for the first time. The test strip includes all the necessary components for analysis (membranes, protein antigen conjugates, colored nanoparticles), except specific free unconjugated antibodies that are transferred to the buffer used for sample dilution. Thus, the concentrations of the antibodies and markers can be chosen independently, and dilution of the sample with buffer (a routine procedure for any immunochromatographic test) ensures pre-incubation of the antibodies with the sample. Both factors increase the sensitivity of the test. The proposed scheme has been proven to be effective for the determination of deoxynivalenol and T-2 toxin; detection limits of 500 pg/mL and 50 pg/mL, respectively, were achieved. This is an order of magnitude lower than the limits of detection of traditional tests using the same reagents. The analysis was conducted in water organic extracts (20% methanol); the duration of the analysis is 10 min (it's the same time as for a traditional test). The proposed approach is universal, and it can be used to detect a variety of compounds.
外文关键词：Immunochromatography；Test strip；Immunosensor；Gold nanoparticles；Indirect labeling；Sensitivity enhancement
作者：Petrakova, AV；Urusov, AE；Gubaydullina, MK；Bartosh, AV；Zherdev, AV；Dzantiev, BB；AF Petrakova, Alina V.；Urusov, Alexandr E
作者单位：Russian Acad Sci
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：-
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