中文摘要：本研究原位合成了选择性识别黄曲霉毒素B1的纳米结构聚合物膜，并用作改良的荧光传感器的高灵敏度识别元件。还用该改良技术成功地分析了模型溶液及面包厂废水。
外文摘要：Nanostructured polymeric membranes for selective recognition of aflatoxin B1 were synthesized in situ and used as highly sensitive recognition elements in the developed fluorescent sensor. Artificial binding sites capable of selective recognition of aflatoxin B1 were formed in the structure of the polymeric membranes using the method of molecular imprinting. A composition of molecularly imprinted polymer (MIP) membranes was optimized using the method of computational modeling. The MIP membranes were synthesized using the nontoxic close structural analogue of aflatoxin B1, ethyl-2-oxocyclopentanecarboxylate as a dummy template. The MIP membranes with the optimized composition demonstrated extremely high selectivity towards aflatoxin B1 (AFB1). Negligible binding of close structural analogues of AFB1 - aflatoxins B2 (AFB2), aflatoxin G2 (AFG2), and ochratoxin A (OTA) was demonstrated. Binding of AFB1 by the MIP membranes was investigated as a function of both type and concentration of the functional monomer in the initial monomer composition used for the membranes' synthesis, as well as sample composition. The conditions of the solid-phase extraction of the mycotoxin using the MIP membrane as a stationary phase (pH, ionic strength, buffer concentration, volume of the solution, ratio between water and organic solvent, filtration rate) were optimized. The fluorescent sensor system based on the optimized MIP membranes provided a possibility of AFB1 detection within the range 14-500 ng mL(-1) demonstrating detection limit (36) of 14 ng mL(-1). The developed technique was successfully applied for the analysis of model solutions and waste waters from bread-making plants.
外文关键词：Nanostructured polymers；Molecularly imprinted polymeric membranes；Sensors；Fluorescent chemo-sensing；Mycotoxins；Aflatoxin B1
作者：Sergeyeva, T；Yarynka, D；Piletska, E；Lynnik, R；Zaporozhets, O；Brovko, O；Piletsky, S；El'skaya, A；AF Sergeyeva, Tetyana
作者单位：Inst Mol Biol & Genet
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：-
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