中文摘要：本研究研发并评估了一种基于发光二极管和光电二极管的简便、高灵敏微荧光计。它采用发光二极管（LED）作光源，比色皿作检测单元，并用光电二极管（PD）作光电探测器。基于该基本结构，用低能DV LED为光源，研发了一种便携荧光计，其检测黄曲霉毒素B1的LOD为0.1ppb。
外文摘要： A facile and high sensitive micro fluorimeter was developed and evaluated. It employed light emitting diode (LED) as light source, cuvette as detection cell, and photodiode (PD) as optoelectronic detector. Optical and electronic parameters were optimized and demonstrated. A high power LED was chosen, which could irradiate the inner area of the cuvette completely at the same time with divergence angle as small as possible. The optimum LED brought 2.5 times signal-to-noise ratio (SNR) enhancement. Using reflector at the opposite direction of excitation light path doubled SNR. The amplifier circuit of PD was deeply investigated to achieve high sensitivity, low noise, and good stability. The limit of detection (LOD) of fluorescein isothiocyanate (FITC) and chlorophyll at SNR = 3 were 10 pM similar to 0.004 ppb and 0.05 ppb, respectively. Basing on the principle structure, a portable fluorimeter for fungimycin detection was developed using a low power DV LED as light source. The LOD for aflatoxin B1 was 0.1 ppb.
外文关键词：Micro fluorimeter；Light emitting diode；Photodiode；High sensitive；Amplifier
作者：Geng, XH；Gao, Y；Feng, CB；Guan, YF；AF Geng, Xuhui；Gao, Yan；Feng, Chunbo；Guan, Yafeng
作者单位：Chinese Acad Sci
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：-
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