中文摘要：本研究开发了一种使用纳米抗体（Nb），快速、定性、视觉检测谷物中赭曲霉毒素A（OTA）的基于聚偏氟乙烯膜的斑点免疫测定法。研究表明该方法是一种有用、无需特殊仪器、现场快速筛查谷物中OTA的工具。
外文摘要：A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (Nb) for rapid, qualitative, and visual detection of ochratoxin A (OTA) in cereals was developed. On the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 mu g/kg for OTA, and the final results were obtained within 20 min. This method was simple with no time-consuming cleanup procedure. Good accuracy and reproducibility were obtained in recovery experiments. Results of the Nb-based dot ELISA are in agreement with the ELISA kit, except for samples that were negative for OTA presence assessed through Nb-based dot ELISA but found positive through the ELISA kit. These results indicated that the developed method could be a useful on-site screening tool for rapid detection of OTA in cereals without special instrument.
外文关键词：Ochratoxin A；Nanobody；Cereal；Dot ELISA；Polyvinylidene fluoride membrane
作者：Sun, ZC；Duan, ZH；Liu, X；Deng, X；Tang, ZW；AF Sun, Zhichang；Duan, Zhenhua；Liu, Xing；Deng, Xing；Tang, Zongwen
作者单位：Hezhou Univ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：11
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