中文摘要：本研究开发了一种同时定量检测乳品中五种黄曲霉毒素（B1、B2、G1、G2及M1）的高效液相色谱结合荧光检测(HPLC-FLD)的分析方法。研究表明，鉴于其简单的预处理、快速测定及高灵敏度，该量化方法能成功用于确定和量化复杂基质中的黄曲霉毒素成份。
外文摘要：An analytical method based on high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was developed for the simultaneous quantification of five aflatoxins (B-1, B-2, G(1), G(2), and M-1) in dairy products. The extraction and HPLC separation parameters of the analytes were optimized. The ultrasonically homogenized samples were extracted using 84% (v/v) acetonitrile aqueous solution. The samples were purified using a Mycosep (R) 226 Aflazon+ multi-functional purification column. After filtration and concentration, the analytes were separated using a DIKMA Diamonsil-C-18(2) column (4.6 mm x 150 mm, 5 mu m) and then eluted with methanol/acetonitrile (4 : 7)-water for analysis. Aflatoxins were separated by elution with a retention time of 20 min. The LOQ of selected analytes ranged from 0.07 mu g kg(-1) to 0.43 mu g kg(-1). Moreover, high correlation coefficients (r(2) > 0.999) were obtained for the five aflatoxins within the respective linear range of 0.06-40.6 ng mL(-1). In addition, the recovery rate was within 82.8-104.5%. Given its simple pretreatment, rapid determination, and high sensitivity, the proposed quantitative method can be successfully used to determine and quantify aflatoxin contaminants in complex matrices.
作者：Xing, YN；Ouyang, QF；Wang, SK；Zhou, XY；AF Xing, Yanan；Ouyang, Qiaofeng；Wang, Shaokun；Zhou, Xiaoying
作者单位：Xinjiang Normal Univ
期刊名称：NEW JOURNAL OF CHEMISTRY
期刊影响因子：3.277
出版年份：2017
出版刊次：18
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