中文摘要：黄曲霉毒素是由真菌黄曲霉及寄生曲霉产生的促癌天然毒素。黄曲霉毒素因其对人和动物的强肝毒、强致癌及强致畸作用，被视为食品安全最严重的威胁之一。这些毒素中，黄曲霉毒素B1（AFB1）是一个备受关注的常见类型。因此，开发一种快速 、简便而可靠的方法检测食品中的AFB1非常重要。本文描述了一种检测AFB1的无镉量子点荧光共振能量转移(FRET)免疫分析法的初步研究。结果表明，在试管中检测AFB1的临界值为10 ng/mL。与之前报道的结合Cd量子点的FRET分析法相比，改良后的FRET操作方便、直观安全。
外文摘要：Aflatoxins are cancer-promoting natural toxins that are produced by the fungus Aspergillus flavus and Aspergillus parasiticus. Aflatoxins have been regarded as one of the most fatal threat in food safety, due to their strong hepatotoxic, carcinogenic and teratogenic effects on human beings and animals. Among them, aflatoxin B1 (AFB1) is one of the common types which have received considerable attention. Thus, developing a rapid, simple and reliable method for determination of AFB1 in foods is very important. Herein, a preliminary study of Frster resonance energy transfer (FRET) immunoassay based on the cadmium-free quantum dots for determination of AFB1 was described. To avoid the use of hazardous heavy metals, core/shell InP/ZnS quantum dots (QDs) as an alternative for Cd-based QDs were synthesized. A silica shell with epoxy groups was used for water solubilization of the obtained nanoparticles. Then a specific anti-AFB1 monoclonal antibody (mAb) was labelled with the hydrophilic QDs via these highly reactive epoxy groups. Gel electrophoresis was used to control the binding. After that, the FRET system was developed using the Cd-free QDs conjugate as donor. Graphene oxide was selected as acceptor. In order to keep the distance between donor and acceptor close enough, the size of silica coated QDs should be controlled strictly. We found that 1-dodecantiol which was used for ligands change on the surface of InP/ZnS QDs was better than oleylamine and the optimum amount of tetraoxysilane was 5 mu L in the silylanization. Besides, only ethanol and hexane were used to wash silica coated QDs which could ensure good dispersion of QDs in water. The cut-off value for the determination of AFB1 in tube was 10 ng/mL with a preliminary study. Compared to reported FRET assays with Cd-based QDs, the developed FRET was easy-to-operate, visual and safe.
作者：De Saeger, S；Logrieco, A；AF De Saeger, Sarah；Logrieco, Antonio
作者单位： Univ Ghent
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：9
点击下载：来自2017年9月11-14日比利时根特举办的第一届MYCOKEY全球减少食品及饲料链中霉菌毒素国际会议的报道