中文摘要：植物源化合物在降低食物和饲料中的霉菌毒素负荷方面已成为一个迅速发展的研究领域，对植物育种和寻找天然杀菌剂具有重要意义。本研究将固体YES培养基上产毒的黄色镰刀菌和禾谷镰刀菌暴露于与麦麸中所报道的水平相近的芥子酸。研究表明外源芥子酸减少了镰刀菌产生的酚酸的积累量；并减少了真菌产生的单端孢霉烯毒素。
外文摘要：Plant-derived compounds for reducing the mycotoxin load in food and feed have become a rapidly developing research field of importance for plant breeding efforts and in the search for natural fungicides. In this study, toxigenic strains of Fusarium culmorum and F. graminearum sensu stricto were exposed to sinapic acid on solid YES media at levels close to those reported in wheat bran. Fusaria produced phenolic acids, whose accumulation was decreased by exogenous sinapic acid. Strains exposed to the lowest doses of sinapic acid showed more efficient reduction of phenolic acid production than fungi kept at higher concentrations of this compound. Fungi reduced exogenous sinapic acid, leading to the formation of syringic aldehyde. Treatment with sinapic acid led to a dramatic accumulation of its parent compound ferulic acid, presumably due to inhibition of the further conversion of this phenolic compound. Exogenous sinapic acid decreased the production of trichothecenes by fungi. Higher doses of sinapic acid resulted in more efficient reduction of mycotoxin accumulation in the media. Gene expression studies of Tri genes responsible for trichothecene biosynthesis (Tri4, Tri5 and Tri10) proved that the inhibition of mycotoxin production by sinapic acid occurred at the transcriptional level. Fusaria respond to sinapic acid by stimulation of ergosterol biosynthesis.
外文关键词：Fusarium；trichothecenes；sinapic acid
作者：Kulik, T；Stuper-Szablewska, K；Bilska, K；Busko, M；Ostrowska-Kolodziejczak, A；Zaluski, D；Perkowski, J；AF Kulik, Tomasz；Stuper-Szablewska, Kinga
作者单位：Univ Warmia & Mazury
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：9
点击下载：芥子酸影响黄色镰刀菌及狭义禾谷镰刀菌的酚醛树脂及单端孢霉烯毒素成分