中文摘要：黄曲霉、寄生曲霉和杂色曲霉产生的黄曲霉毒素和杂色曲霉素是食品和饲料中常见的霉菌毒素。杂色曲霉素是黄曲霉毒素生物合成的直接前体。本研究标准化了使用TaqMan化学探针进行实时PCR扩增曲霉属的黄曲霉毒素/杂色曲霉素生物合成基因omt-1。结果清楚地显示，实时PCR技术能有效地将产毒的寄生曲霉、杂色曲霉和黄曲霉菌株从通常寄居在饲料中的其他霉菌中区分开来。
外文摘要：Aflatoxin and sterigmatocystin produced by Aspergillus flavus, A. parasiticus and A. versicolor are common mycotoxins found in food and feed stuffs. Sterigmatocystin is a direct precursor in aflatoxin biosynthesis. In the present study, Real time PCR using TaqMan probe chemistry was standardized for amplification of the aflatoxin/sterigmatocystin biosynthesis gene omt-1 of Aspergillus spp. The gene was amplified in six cultures of Aspergillus spp. isolated from 53 aflatoxin positive feed samples hence indicating their aflatoxigenic potential. Data clearly revealed that the real time PCR technique is efficient in distinguishing toxigenic strains of A. parasiticus, A. versicolor and A. flavus from other molds commonly inhabiting the feed.
外文关键词：Aflatoxin；Aspergillus flavus；Aspergillus parasiticus；Aspergillus versicolor；Real time PCR；Sterigmatocystin
作者：Singh, R；Rai, TS；Sharma, NS；Arora, AK；Kaur, P
作者单位：Guru Angad Dev Vet & Anim Sci Univ
期刊名称：INDIAN JOURNAL OF ANIMAL RESEARCH
期刊影响因子：0.091
出版年份：2017
出版刊次：4
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