中文摘要：脱氧雪腐镰刀菌烯醇（DON）是属于B组单端孢霉烯霉菌毒素。由于其对人体健康的不利影响，几乎所有的色谱方法都曾用于对DON进行分析和检测。本研究首次报道了应用HPTLC/MS确定小麦作物中DON的存在。结果证明智利小麦样品中确实存在DON。
外文摘要：Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON in wheat crops.Chromatography was performed on silica gel 60 F-254 HPTLC plates using toluene-ethyl acetate - formic acid 6: 3: 1 (V/V/V) as mobile phase. After post-chromatographic derivatization with 10 % AlCl3, fluorescence detection was carried out at UV 366/> 400 nm. Bands identity and purity was confirmed by mass spectrometry acquiring analytes directly from the sample bands by means of TLC/MS elution head-based interface. Both, sample and standard bands showed clear mass signals at m/z 341 that corresponds to the formate adduct of DON [M+HCOO](-). Thus, the presence of DON in the Chilean wheat sample was confirmed.
外文关键词：Deoxynivalenol；mycotoxins；HPTLC/MS；Fusarium；TLC
作者：Vega-Herrera, M；Madariaga, R；Aranda, M；Morlock, GE
作者单位：Justus Liebig Univ Giessen
期刊名称：JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
期刊影响因子：0.429
出版年份：2017
出版刊次：2
点击下载：通过高效薄层色谱-质谱确认智利小麦中脱氧雪腐镰刀菌烯醇的存在