对总黄曲霉毒素ELISA检测试剂盒Veratox(R)的MAX水相萃取进行验证

Validation of MAX Aqueous Extraction on Veratox (R) for Total Aflatoxin ELISA Test Kit

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中文摘要:Neogen公司(美国密歇根州兰辛市)开发了一种普通的水相萃取方法用于ELISA或侧流法检测霉菌毒素。总黄曲霉毒素ELISA提取的Veratox(R)使用MAX 2提取包和水代替传统使用的有机溶剂。该试剂盒已得到谷物检验、包装公司和畜牧业管理局的批准。
外文摘要:Neogen Corp. (Lansing, MI) has developed a common aqueous extraction method for the detection of mycotoxins in the ELISA or lateral flow format. The Veratox (R) for Total Aflatoxin ELISA extraction method uses a MAX 2 extraction packet and water in replacement of traditionally used organic solvents. Veratox (R) for Total Aflatoxin has a detection range of 5-50 ppb neat or up to 300 ppb with dilution. The kit development focused on superior cross reactivity, ability to accurately detect naturally contaminated samples, and utilization of an aqueous extraction method. In two separate validation studies, the Veratox (R) for Total Aflatoxin test kit resulted in average yields of 91-114% in naturally contaminated mycotoxin reference material corn. The cross-reactivity profiles for aflatoxins B-1, B-2, G(1), and G(2) were 100, 113, 103, and 93%, respectively. This kit is approved by the Grain Inspection, Packers, and Stockyards Administration.
外文关键词:HEPATOCELLULAR-CARCINOMA;ASPERGILLUS-TAMARII;OCHRATOXIN;OUTBREAK;RISK
作者:Roman,BE;Driksna,D;Abouzied,MM;Klein,F
作者单位:Neogen Corp
期刊名称:JOURNAL OF AOAC INTERNATIONAL
期刊影响因子:0.918
出版年份:2017
出版刊次:4
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  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2017-11-23