中文摘要：本研究用离体实验评估了酿酒酵母吸附伏马菌素B1（FB1）的能力。还对受FB1污染的玉米营养的消化以及消化酶和pH对溶液中的FB1生物利用度的影响进行了评估。研究表明，在这些测定中FB1和酿酒酵母（菌株4）的存在对玉米营养素的消化没有影响。对于酵母的吸附能力，则是当处理玉米籽粒的FB1浓度更高时更为明显。酿酒酵母菌株4去除了溶液中8%-18%的FB1，显示其在马酶消化离体条件下吸附FB1的吸附能力有限。
外文摘要：In this study, the capacity of Saccharomyces cerevisiae to adsorb fumonisin B-1 (FB1) was evaluated in in vitro assays. The digestion of nutrients from maize contaminated with FB1 was assessed as well as the influence of digestive enzymes and pH on the bioavailability of FB1 in solution. Adsorption assays in buffers containing 5 mu g/ml of FB1 were conducted to determine the strain to be used in the in vitro digestion assays. Four different yeast strains (1, 2, 3 and 4) along with five different cell concentrations of each one were studied under pH 2 and 6.8 at 39 degrees C. Strain 4 showed higher adsorption values at 1x10(9) cfu/ml, adsorbing 39.4% of the mycotoxin at pH 2 and 37.5% at pH 6.8. After that, the in vitro enzymatic digestion was conducted in two separated experiments. First, maize artificially contaminated with FB1 (5 mu g/g) was used in five different treatments. Then, assays with maize naturally contaminated with FB1 (Maize A: 3.2 mu g/g and Maize B: 29.0 mu g/g) were conducted. In all samples, FB1 was quantified by HPLC-FL in liquid fraction and in solid residue. Samples of maize in natura and solid residues were subjected to chemical analysis of dry matter, organic matter, crude protein, neutral detergent fibre and starch to estimate the digestion of nutrients. The presences of FB1 and S. cerevisiae (Strain 4) in these assays had no influence on the digestion of the maize nutrients. The adsorption capacity of yeast was observed more clearly in treatments with higher concentrations of FB1 in the maize grain. S. cerevisiae strain 4 removed between 8 to 18% of FB1 in solution, showing a limited capacity to adsorb FB1 under in vitro conditions of horse enzymatic digestion.
外文关键词：adsorption；equine；in vitro digestion；leukoencephalomalacia；yeast
作者：Taran, F. M. P.；Silva, V. P.；Abrunhosa, L.
作者单位：Univ Fed Rural Rio de Janeiro
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2017
出版刊次：2
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