中文摘要：黄曲霉毒素B1（AFB1）和其代谢物黄曲霉毒素M1（AFM1）是众所周知的危害人和动物健康的致癌物。本研究对一种超高效液相色谱联用荧光检测（HPLC-FLD）方法进行了优化和验证。此外，还首次研究了姜黄素对肉仔鸡肝脏、肾脏和肌肉组织中AFB1和AFM1残留物清除的影响，并估算了清除AFB1和AFM1残留物所需的时间。总之，开发的方法适用于商业肉鸡加工企业、食品生产企业和质量控制实验室的质量控制检测。
外文摘要：Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and muscle tissues of broiler chickens and estimated a necessary clearance time required for AFB1 and AFM1 residues. The results showed that the average recoveries of AFB1 varied in liver, kidney, and muscles between 82.32-85.56, 85.34-88.45, and 84.88-89.73% respectively, while the average recoveries of AFM1 in liver, kidney, and muscles varied between 92.17-95.03, 94.12-97.21, and 95.32-98.51%, respectively. The detection limit of aflatoxin B1 was 0.008 ng/ml, while for aflatoxin M1 was 0.003 ng/ml. The limit of quantification (LOQ) for AFB1 and AFM1 was 0.02 and 0.01 ng/ml, respectively. Clearance time for AFB1 and AFM1 residues were analyzed in two experimental groups of broilers. One group fed with dietary AFB1 (5.0 mg/kg feed) and other with curcumin+ AFB1 diet (curcumin; 300 mg/kg feed, AFB1; 5.0 mg/kg feed). AFB1 and AFM1 residues clear ance time was calculated based on LOQ using withdrawal time calculation software (WT1.4). Clearance time analyzed for AFB1 ranged from 11 to 19 days and for AFM1 ranged from 10 to 12 days at 95% confidence level. Interestingly, curcumin supplementation in the diet reduced the clearance time of AFM1 in liver and kidney but not in muscle tissues. Conclusively, the developed method can be appropriately used for the quality control testing of commercial broiler-meat processing companies, food manufacturers, and quality control laboratories.
外文关键词：UPLC-FLD；aflatoxin B1；aflatoxin M1；curcumin；arbor acres broiler；clearance time
作者：Cui, Xiaoxu;Muhammad, Ishfaq;Li, Rui
作者单位：Northeast Agr Univ
期刊名称：FRONTIERS IN PHARMACOLOGY
期刊影响因子：4.418
出版年份：2017
出版刊次：8
点击下载：开发一种检测动物组织中黄曲霉毒素B1和M1含量的HPLC-FLD法，研究姜黄素对霉菌毒素清除率的影响