中文摘要：本研究开发了一种新的免疫层析法用于低分子量分析物的高灵敏度检测。研究表明，本设计测试系统的仪器和视觉检测极限分别是0.1和0.5ng/ml（每克分析物2和90ng），比使用相同试剂的传统免疫色谱法低两个数量级。
外文摘要：A new scheme of immunochromatographic assay was developed for the highly sensitive detection of low-molecular-weight analytes. This scheme includes the following two steps: the formation of complexes of free specific antibodies with an antigen and their detection by anti-species antibodies conjugated to gold nanoparticles as the label. This scheme was tested with mycotoxin T-2 toxin in maize extracts. The use of specific antibodies and a label as two individual components made it possible to independently vary their concentrations with a simultaneous decrease in the detection limit and an increase in the color intensity. The assay did not require additional reagents and manipulations. The instrumental and visual detection limits of the designed test system were 0.1 and 5.0 ng/mL, respectively (2 and 90 ng per gram of analytes), which are two orders of magnitude lower compared to conventional immunochromatography using the same reagents.
外文关键词：immunochromatographic assay；test strips；sensitivity enhancement；gold nanoparticles；T-2 toxin
作者：Urusov, A. E.；Petrakova, A. V.；Bartosh, A. V.
作者单位：Russian Academy of Sciences
期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY
期刊影响因子：0.671
出版年份：2017
出版刊次：5
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