中文摘要：如今免疫亲和柱（IACs）可用于所有主要的霉菌毒素。然而制造商通常不提供或仅提供非常有限的抗原表位信息，即毒素分子与抗体结合的特定部分。4-脱氧雪腐镰刀菌烯醇（DON）是一种单端孢霉烯族毒素，由植物病原田间真菌产生，在全球许多国家受到管制。研究显示，DON通过不同的代谢途径进行生物转化，从而可在不同的产品或有机体中发现其不同的生物转化产物。此外，几种结构相似的霉菌毒素可与DON共现。本研究比较了五种IACs对一系列的DON C-3、C-8、C-10、C-13 或C-15衍生物以及雪腐镰刀菌烯醇（NW）的保留情况。结果表明，IACs中所有抗体都能结合一系列的DON衍生物，特别是C-3衍生物。三种柱子能保留NW，而仅一种柱子能保留部分T-2四醇。
外文摘要：Commercial immunoaffinity columns (IACs) are today available for all major mycotoxins. However, manufacturers give usually no or very limited information on the epitope, i.e. the specific part of the toxin molecule that binds to the antibody. 4-Deoxynivalenol (DON) is a trichothecene mycotoxin that is produced by plant pathogenic field fungi and is regulated in many countries worldwide. DON was shown to be biotransformed via different metabolic pathways, and thus many different biotransformation products may be found in different products or organisms. In addition, several structurally similar mycotoxins may co-occur with DON. We compared five commercial IACs for their retention of a range of DON derivatives modified in the C-3, C-8, C-10, C-13 or C-15 positions, as well as nivalenol (NW) and T-2 tetraol. The DON-derivatives were deepoxy-DON, DON 3-, 8- and 15-O-beta-D-glucuronides, 3- and 15-O-acetyl-DON, DON-3-O-beta-D-glucoside, 10- and 13-cysteinyl-adducts of DON, and the 13-mercaptoethanol and 10,13-dimercaptoethanol adducts of DON. The C-3 derivatives and deepoxy-DON were retained by most of the columns. Only one of the five IACs retained C-15 and C-8 derivatives, but it did not retain C-3 derivatives or deepoxy-DON. The antibodies in two of the IACs bound C-10 conjugates, but C-13 derivatives were not retained by any of the columns. This study shows that all of the antibodies in commercial IACs bind a range of DON derivatives, especially those that are modified at C-3. NW was retained by three of the columns, and T-2 tetraol was partially retained by one IAC.
外文关键词：Deoxynivalenol；Antibody；Immunoaffinity chromatography；Trichothecene
作者：Uhlig, S；Stanic, A；Hussain, F；Miles, CO；AF Uhlig, Silvio；Stanic, Ana；Hussain, Fozia；Miles, Christopher O.
作者单位：Norwegian Vet Inst
期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
期刊影响因子：2.687
出版年份：2017
出版刊次：-
点击下载：免疫亲和柱对霉菌毒素隐蔽型4-脱氧雪腐镰刀菌烯醇（DON）的选择性