中文摘要：先前已证明动物日粮中添加钙蒙脱石粘土(Novasil)，能结合黄曲霉毒素B1（AFB1）并减轻黄曲霉毒素中毒症状。为了探究并改善粘土材料吸附黄曲霉毒素的能力和功效，本研究研发并测试了用营养素包括L-肉碱和胆碱改良的钙和钠蒙脱石粘土。还用等温分析确定了这些改良吸附剂对AFB1的吸附作用，并测试了它们对成年水螅AFB1中毒的保护能力。结果表明肠道吸附剂疗法可以在突发和爆发急性黄曲霉毒素中毒时，添加到易受黄曲霉毒素污染的营养补充剂、食品、风味饮料和动物饲料中，并作为筛选危险环境化学品的模型。
外文摘要：Previously, a calcium montmorillonite clay (Novasil) included in the diet of animals has been shown to bind aflatoxin B1 (AfB1) and reduce the symptoms of aflatoxicosis. To investigate and improve the capacity and efficacy of clay-based materials as aflatoxin sorbents, we developed and tested calcium and sodium montmorillonite clays amended with nutrients including L-carnitine and choline. Also, we determined the sorption of AfB1 by isothermal analysis and tested the ability of these amended sorbents to protect adult hydra from AfB1 toxicity. The results showed that exchanging montmorillonite clays with L-carnitine and choline inhibited swelling of the clays and increased the sorption capacity and efficacy of clay surfaces for AfB1. Results from dehydroxylated and heat-collapsed clays suggested that AfB1 was primarily adsorbed in the clay interlayer, as predicted from thermodynamic calculations and computational modeling. The hydra bioassay further indicated that the modified clays can significantly protect adult hydra from AfB1 with as low as 0.005% clay inclusion. This enterosorbent therapy may also be applied to screen hazardous chemicals such as pesticides and PAHs based on similar sorption mechanisms. Taken together, enterosorbent therapy could be delivered in nutritional supplements, foods that are vulnerable to aflatoxin contamination, flavored liquids and animal feeds during emergencies and outbreaks of acute aflatoxicosis, and as a screening model for hazardous environmental chemicals.
作者： Wang, MC；Maki, CR；Deng, YJ；Tian, YN；Phillipe, TD；AF Wang, Meichen；Maki, Cody R.；Deng, Youjun；Tian, Yanan；Phillipe, Timothy D.
作者单位：Texas A&M Univ
期刊名称：CHEMICAL RESEARCH IN TOXICOLOGY
期刊影响因子：3.025
出版年份：2017
出版刊次：9
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