中文摘要：黄曲霉和寄生曲霉是粮食作物（玉米、棉花、花生和油籽）已知的产黄曲霉毒素的主要污染物。目前，通过开发并运用各种改良的栽培措施来克服食品污染问题。本研究旨在通过有毒食品平板技术及MTT试验(3-(4,5-二甲基噻唑-2-yl)-2,5-二苯基四氮唑溴化物)评估植物化学物质（抗坏血酸、没食子酸、咖啡因和槲皮素）对产毒黄曲霉(MTCC 11866)和寄生曲霉(MTCC 8189)菌株的作用。与对照（无植物化学物质添加的曲霉孢子）相比，发现所有化合物都能显著减缓真菌的生长。
外文摘要：Aspergillus flavus and Aspergillus parasiticus are the major contaminants known to produce aflatoxins in food crops (maize, cotton, corn, peanuts and oil-seed). Nowadays various modified cultural practices are being utilized and explored to overcome food contamination problem. The current study was aimed to evaluate the effect of phytochemicals (Ascorbic acid, Gallic acid, Caffeine and Quercetin) on toxigenic strains of A. flavus (MTCC 11866) and A. parasiticus (MTCC 8189) by Poisoned food plate technique and MTT assay (3-(4,5-Dimethylthiazole-2-yl)-2,5-Diphenyltetrazolium bromide). In comparison with control (Aspergillus spores without phytochemicals) a significant decrease in growth was observed with all the compounds. Quercetin showed strongest inhibitory effect with MIC50 at 36 A mu g/ml in A. parasiticus and MIC50 at 113 A mu g/ml in A. flavus. Caffeine showed good inhibitory effect in A. parasiticus (160 A mu g/ml) whereas Gallic acid showed good inhibitory effect in A. flavus (153 A mu g/ml). Ascorbic acid showed least effect among phytochemicals tested in both the Aspergillus species (322 A mu g/ml).
外文关键词：Phytochemicals；Antifungal；Aspergillus flavus；Aspergillus parasiticus；MTT；Poisoned food technique
作者：Tiwari, S；Gupta, N；Malairaman, U；Shankar, J；AF Tiwari, Shraddha；Gupta, Nupur；Malairaman, Udayabanu；Shankar, Jata
作者单位：Jaypee Univ Informat Technol
期刊名称：NATIONAL ACADEMY SCIENCE LETTERS-INDIA
期刊影响因子：0.345
出版年份：2017
出版刊次：4
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