中文摘要：本研究以黄曲霉毒素B1（AFB1）为例，展示了一种集成的智能手机应用芯片（SPAC）系统，用于现场定量测定十亿分之几（ppb）水平的食物毒素。研究认为，该集成SPAC系统有望成为一种简单而精确的食物毒素定量方法，为现场快速筛选带来大量好处。
外文摘要：We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional enzyme-linked immunosorbent assay (ELISA) kits were obtained. The achieved detection limit (3 +/- 1 ppb, equivalent to mu g/kg) and dynamic response range (0.5-250 ppb) meet the requested testing standards set by authorities in China and North America. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.
作者：Li, Xiaochun；Yang, Fan；Wong, Jessica X. H
作者单位：Taiyuan Univ Technol
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：17
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