中文摘要：由麦角菌产生的麦角生物碱霉菌毒素是谷类作物和牧草的污染物。本研究的目的是确定麦角菌硬粒数量和重量相比于总麦角生物碱浓度之间的相关性，利用高效液相色谱-串联质谱法评估研磨过程（即粒度（PS））对麦角生物碱分析的影响，并确定样品量对分析变异性的影响。研究表明，在麦角碱浓度低于350aeg/kg时，用麦角菌硬粒数量和重量作为生物碱含量的指标是不可靠，且颗粒大小影响变异性。大样本精细研磨（平均PS <200 mu m，85％颗粒<400 mu m）的分析方法应该用来评估低水平的麦角碱污染。
外文摘要：Ergot alkaloid mycotoxins produced by the fungus Claviceps purpurea, are contaminants of cereal crops and grasses. The objectives of this study were to determine the correlation between number of ergot sclerotia and weight compared to the total ergot alkaloid concentration, to evaluate the effect of grinding process (i.e. particle size (PS)) on ergot alkaloid analysis using high performance liquid chromatography - tandem mass spectrometry, and to determine the impact of sample volume on analytical variability. This study demonstrated that correlations exist between both ergot sclerotia count (R-2=0.7242, P<0.001) and ergot sclerotia weight (R-2=0.9618, P<0.001) compared to the total alkaloid concentration of 6 ergot alkaloids. However, at alkaloid ergot concentrations below 350 aeg/kg grain, ergot sclerotia count (R-2=0.0002, P=0.956) and ergot sclerotia weight (R-2=0.0064, P=0.769) were not correlated to the total alkaloid concentration. A lower variability (P=0.041), defined by coefficient of variation (CV), was observed using a commercial UDY cyclone sample mill (PS=192 mu m, CV= 9 aeg/kg) as compared to a household coffee grinder (PS=516 mu m, CV= 66 aeg/kg). Total amount and concentration of individual ergot alkaloids varied (P<0.05) among sclerotia of similar weight. For the analytical method, CV was numerically reduced as sample volume increased (97% CV for 75 ml to 64% CV for 1000 ml; mean of all concentrations) but increased as sample concentration declined (17% CV for 81,678 mu g/kg to 284% for 35 mu g/kg; mean of all sample volumes). This implies that analysis of small sample volumes at low ergot alkaloid concentrations may result in highly variable and potentially misleading results. In conclusion, number of ergot sclerotia and weight are unreliable indicators of alkaloid content at ergot concentrations below 350 aeg/kg and particle size influences the variability. An analytical approach with fine grinding (mean PS<200 mu m, 85% particles <400 mu m) of a large sample should be used to assess low-level ergot contamination.
外文关键词：sclerotia；sample volume；particle size
作者：Grusie, T.；Cowan, V.；Singh, J.
作者单位：University of Saskatchewan
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2017
出版刊次：3
点击下载：确定谷物麦角碱(Claviceps purpurea)污染的称重、计数和分析方法之间的相关性和变异性