中文摘要：Diospyros mafiensis F. White是一种药用灌木或小树（高6米），广泛分布于桑给巴尔-伊尼扬巴内地区，坦桑尼亚和莫桑比克传统上用来治疗麻风、腹泻和皮肤真菌感染。本研究的目的是确定D. mafiensis根皮成分抑制黄曲霉和寄生曲霉的生长、孢子形成和黄曲霉毒素产生的抗产毒性质。研究表明，DQ（二醌）和3HQ（3-羟基丙醌）可作为天然抗真菌化合物，防止食物和饲料中霉菌的生长和黄曲霉毒素的积累。
外文摘要：Diospyros mafiensis F. White is a medicinal shrub or small tree (6 m tall) widely distributed in the Zanzibar-Inhambane regional mosaic and traditionally used to treat leprosy, diarrhoea, and skin fungal infections in Tanzania and Mozambique. Our objective was to determine the anti-aflatoxigenic properties of compounds from D. mafiensis root bark against vegetative growth, sporulation and aflatoxin production by Aspergillus flavus and Aspergillus parasiticus. Bioassay-guided extraction, fractionation, and isolation of bioactive compounds using A. parasiticus B62 were employed. The bioactive compounds were elucidated using H-1 and (CNMR)-C-13 and LC-MS. Growth inhibition was determined by measuring the colony diameter of A. flavus AF3357 and A. parasiticus SU-1 ATCC56775. Inhibitory effects on sporulation were estimated using a haemocytometer. Total aflatoxin was quantified by direct competitive enzyme-linked immunosorbent assay (ELISA). Bioactive compounds diosquinone (DQ) and 3-hydroxydiosquinone (3HDQ) were identified. DQ weakly inhibited A. flavus and A. parasiticus vegetative growth (MIC50 > 100 mu g/ml) and 3HDQ strongly inhibited A. flavus (MIC50 = 14.9 mu g/ml) and A. parasiticus (MIC50 = 39.1 mu g/ml). DQ strongly reduced total aflatoxin production by A. flavus from 157 to 36 ng/plate, and by A. parasiticus from 1,145 ng/plate to 45 ng/plate at 100 mu g/ml. 3HDQ reduced total aflatoxin production by A. parasiticus from 1,145 to 32 ng/plate; stimulated production by A. flavus from 157 to 872 ng/plate at 12.5 mu g/ml but reduced to 45 ng/plate at 100 mu g/ml. In summary, DQ and 3HDQ could be used as natural antifungal compounds to prevent mould growth and aflatoxin accumulation in food and feed.
外文关键词：diosquinone；3-hydroxydiosquinone；total aflatoxin；A. flavus；A. parasiticus
作者：Mmongoyo, J. A.；Nair, M. G.；Linz, J. E.
作者单位：Michigan State University
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2017
出版刊次：3
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