中文摘要：本研究开发了一种简便快捷而经济的QuEChERS法，用于ELISA检测谷物中的ZEN和DON。本研究报道的QuEChERS提取接酶联免疫吸附测定法检测谷物中的ZEN及DON尚属首次。
外文摘要：A simple, rapid and cost-effective QuEChERS method for the determination of ZEN and DON by ELISA in cereals was developed. The single factor experiment was used to find the optimal QuEChERS parameters (extraction solvent, extraction method, extraction time, the QuEChERS composition, sorbent and clarification time) and extracts were analysed by ELISA. The optimised method was validated for selectivity, linearity, limits of detection, matrix effect and precision. The recovery values of ZEN and DON by ELISA were compared with a standard HPLC method and the results showed good coefficient with HPLC, which proved to be suitable for the screening of cereal samples for the presence of ZEN or DON. This is the first report of the determination of ZEN and DON in cereals after QuEChERS extraction combined with the enzyme-linked immunosorbent assay method.
外文关键词：Zearalenone；deoxynivalenol；QuEChERS；ELISA；cereals
作者： He, QR；Peng, HW；Yang, JY；Xu, ZL；Fan, CC；Sun, YM；AF He, Qiurong；Peng, Hongwei；Yang, Jinyi；Xu, Zhenlin；Fan, Congcong；Sun, Yuanming
作者单位：South China Agr Univ
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY
期刊影响因子：1.548
出版年份：2017
出版刊次：6
点击下载：QuEChERS提取接酶联免疫吸附测定法检测谷物中的脱氧雪腐镰刀菌烯醇与玉米赤霉烯酮