中文摘要：由于转基因作物受到全球的重视，因此其批准和商业化就需要准确可靠的转基因作物检测方法。目前检测技术主要分为检测转基因生物及其产品中转基因（1）DNA和（2）蛋白质两类。常规方法如PCR（聚合酶链反应）和酶联免疫吸附试验（ELISA）分别用于DNA和蛋白质定量分析。尽管这些技术（PCR和ELISA）非常方便有效，但是随着越来越复杂的转基因生物产品的日益增加，因而需要更先进的技术以便进行转基因作物的高通量检测和定量化。因此，基因芯片、毛细管凝胶电泳、数字PCR和下一代测序等现代技术因其对基因物质含量的准确和精确检测而有望成为新的研究方向。本文依据出现时间、可行性、准确性和成本效益对这些检测技术进行了简短的比较研究。特定转基因品种的检测、不同转基因品种的污染、融合测定以及堆叠的基因蛋白是这些新兴技术未来需要解决的关键问题。
外文摘要：As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1) DNA and (2) proteins from GMOs and their products. Conventional methods such as PCR (polymerase chain reaction) and enzyme-linked immunosorbent assay (ELISA) were routinely employed for DNA and protein based quantification respectively. Although, these Techniques (PCR and ELISA) are considered as significantly convenient and productive, but there is need for more advance technologies that allow for high throughput detection and the quantification of GM event as the production of more complex GMO is increasing day by day. Therefore, recent approaches like microarray, capillary gel electrophoresis, digital PCR and next generation sequencing are more promising due to their accuracy and precise detection of transgenic contents. The present article is a brief comparative study of all such detection techniques on the basis of their advent, feasibility, accuracy, and cost effectiveness. However, these emerging technologies have a lot to do with detection of a specific event, contamination of different events and determination of fusion as well as stacked gene protein are the critical issues to be addressed in future.
外文关键词：GMOs; detection; quantification; digital-PCR; micro array; next generation sequencing
作者：Salisu, Ibrahim B.; Shahid, Ahmad A.; Yaqoob, Amina; 等
作者单位：Fed Univ Dutse
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.298
出版年份：2017
出版刊次：10
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