中文摘要：异色瓢虫在我国是一种常见且丰富的捕食性瓢虫，其通过取食植物植株或捕食靶标及非靶标食草昆虫可能会接触到转Bt基因作物产生的Cry毒素。表达Cry1Ab/Cry2Aj融合蛋白的新型转基因玉米已被研发出来，而对其天敌造成的生态风险应进行严谨的评估。本文分别通过成年异色瓢虫取食转Bt基因玉米花粉和异色瓢虫捕食鳞翅目昆虫研究了转Bt基因玉米对非靶标捕食者异色瓢虫的二级和三级营养影响。甜菜夜蛾初孵幼虫可转移Bt蛋白，且ELISA生物测定表明Bt蛋白可通过转基因玉米-取食昆虫-捕食瓢虫进行稀释转移。捕食转Bt基因玉米和非转基因玉米的异色瓢虫在存活量、发育、重量、产卵量、卵孵化率等生活史参数上均没有明显差异。另外，直接取食转Bt基因玉米花粉对雌雄异色瓢虫成虫的适应性、存活量、重量也没有不利影响。研究结果表明，转cry1Ab/cry2Aj基因玉米对非靶标异色瓢虫不会造成生态风险。
外文摘要：Harmonia axyridis (Pallas) is a common and abundant predator in China and may be exposed to Cry toxins that are produced in Bt crops either by feeding on plant parts or by feeding on target or nontarget herbivorous insects. A new Bt maize line, expressing the Cry1Ab/Cry2Aj fused protein, has been developed and should be rigorously assessed for the ecological risks on the natural enemy. Laboratory experiments were carried out to study the effects of this Bt maize on nontarget predator H. axyridis via bitrophic interaction of adult H. axyridis feeding on Bt maize pollen and tritrophic interaction of H. axyridis consuming the lepidopteran prey. Spodoptera exigua (Hubner) neonate larvae were used to transfer Bt protein because they could survive after ingesting transgenic cry1Ab/cry2Aj maize kernels in the previous study. ELISA bioassays confirmed that the Bt protein could be transferred, but diluted through Bt maize-prey-predator. Life history parameters such as survival, development, weight, fecundity, and egg hatching rate were not significantly different when H. axyridis consumed prey that had been reared on Bt maize compared with prey reared on a nontransformed parental control. Furthermore, feeding directly on Bt maize pollen also had no detrimental effects on fitness, survival, and weight of female and male adults. In conclusion, our results indicate that transgenic cry1Ab/cry2Aj maize poses no ecological risks on the nontarget predator H. axyridis.
外文关键词：transgenic cry1Ab/cry2Aj maize; Harmonia axyridis; nontarget risk assessment; tritrophic interaction; bitrophic interaction
作者：Chang, Xue; Lu, Zengbin; Shen, Zhicheng; 等.
作者单位：Zhejiang Univ
期刊名称：ENVIRONMENTAL ENTOMOLOGY
期刊影响因子：1.601
出版年份：2017
出版刊次：10
点击下载：转cry1Ab/cry2Aj基因玉米对非靶标异色瓢虫（鞘翅目：瓢虫科）的二级和三级营养影响