中文摘要：玉米褪绿斑驳病毒和甘蔗花叶病毒复合感染玉米会引起严重的玉米致死性坏死病，本文通过血清学和分子生物学技术研究引起玉米致死性坏死病的相关病毒，本研究收集到174份有症状的玉米叶样本，通过双抗体夹心酶联免疫吸附试验（ELISA）发现43份样本对玉米褪绿斑驳病毒抗体显示出强烈的反应，仅仅14份样本对甘蔗花叶病毒有清晰的反应；逆转录聚合酶链反应（RT-PCR）技术检测显示大多数样本中均含有玉米褪绿斑驳病毒和甘蔗花叶病毒，对这两种病毒外壳蛋白的遗传多样性分析发现：其与保存在东非基因库中的蛋白具有很高的同源性；系统发育分析发现，病毒分离株也高度相似；总之，本研究确切的证实了在所调查地区玉米褪绿斑驳病毒和甘蔗花叶病毒与玉米致死性坏死病的相关性，并且首次通过几个埃塞俄比亚样本的序列分析提供了遗传变异信息，玉米致死性坏死病在所调研区域非常严重，对控制这种疾病的综合治理方法需求尤为迫切。
外文摘要：Maize Lethal Necrosis Disease (MLND) develops from synergistic co-infection by Sugarcane mosaic virus and Maize chlorotic mottle virus. The disease causes symptoms ranging from leaf tissue mottling and malformed ears to premature plant death. Information on the diversity and transmission of those viruses are limited in Ethiopia. No attempts were made to characterize and identify SCMV and MCMV strains of Oromia and Benishangul-Gumuz regions. The aim of this research was to study MLND responsible viruses via serological and molecular techniques. A total of 174 symptomatic maize leaf samples were collected. Using Double Antibody Sandwich-ELISA (DAS-ELISA), 43 samples showed strong reaction for MCMV antibody and only 14 samples were clearly positive for SCMV. Reverse transcriptase polymerase chain reaction (RT-PCR) technique revealed the presence of MCMV and SCMV in most samples. Analysis of the genetic diversity of MCMV and SCMV by sequencing the coat protein of all isolates shows strong homology with previous East African isolates deposited in the GenBank. The phylogenetic analysis indicated that the virus isolates are highly similar with each other. In conclusion, this study unequivocally confirmed the association of MCMV and SCMV with MLND in the investigated areas and for the first time provided information on the genetic variability by sequence analysis of several Ethiopian samples. Since the risk posed by MLND in the investigated area is high, there is an urgent need for developing integrated management options for controlling the disease.
 
外文关键词：DAS-ELISA, Genetic diversity, Maize, MLND, RT-PCR
作者：Fentahun, Mengistu; Feyissa, Tileye; Abraham, Adane; Kwak, Hae Ryun
作者单位：Ethiopian Inst Agr Res, Natl Agr Biotechnol Res Ctr, POB 249, Holetta, Ethiopia.
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY
期刊影响因子：1.478
出版年份：2017
出版刊次：12
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