中文摘要：本文对可同时检测霍乱弧菌O1和A型肉毒梭菌毒素的多色免疫层析试纸条格式进行了描述，单克隆抗体共轭CdTe量子点会分别产生绿色和橙色的发射光，并使用活性葡聚糖作为交联剂；在光照下，检测线分别在540 nm和570 nm处达到峰值，这种横向流动分析系统可以分别在低至1000 CFU/ml和0.8 ng.mL水平下检测到霍乱弧菌和肉毒杆菌毒素，且该方法可以在不经过任何预处理的情况下，10分钟内完成检测。
外文摘要：The authors describe a multicolor immunochromatographic test strip format for simultaneous detection of Vibrio cholera O1 (Ogawa) and of the Clostridium botulinum toxin type A. Respective monoclonal antibodies were conjugated to CdTe quantum dots (QDs) with green and orange emission color. Activated dextran is used as a cross-linker. The CD labeled antibodies were then covered with denatured reduced BSA (dBSA-QD). Under illumination, the test gives lines with emission peaks at 540 nm and 570 nm, respectively. This lateral flow assay system can detect V. cholerae down to 1000 cfu.mL(-1) levels, and C. botulinum toxin down to 0.8 ng.mL(-1). The method allows assays to performed within 10 min without any sample pretreatment.
外文关键词：Lateral flow assay, Multicolor test strip, Pathogenic bacteria, Food safety, Quantumdots, Activated dextran
作者：Gharaat, Morteza; Sajedi, Reza H.; Shanehsaz, Maryam; Jalilian, Nezam; Mirshahi, Manouchehr; Gholamzad, Mehrdad
作者单位：Tarbiat Modares Univ, Fac Biol Sci, Dept Biochem, POB 14115-154, Tehran, Iran.
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.58
出版年份：2017
出版刊次：12
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