中文摘要：有机磷农药检测对食品安全和环境保护至关重要，本文提出了一个使用透明质酸酪氨酸金纳米粒子作为无酶比色探针检测硫代磷酸酯农药残留的方法，金纳米粒子表面的AuNPs会在强碱性的透明质酸酪氨酸金纳米粒子胶体溶液中使得硫代磷酸酯发生水解，从而影响金纳米粒子的聚集状态并产生红变蓝的颜色变化，该方法设计简单且易操作，仅仅需要在室温下，将待检测物加入到透明质酸酪氨酸金纳米粒子胶体溶液中；该方法对甲拌磷的检测限低至0.005 μg/mL，低于欧盟最大残留限量（MRL），最重要的是该方法可直接应用于实际产品的甲拌磷残留检测。
 
外文摘要：Detection of organophosphorus pesticides (OPPs) is crucial for food safety and environmental protection. Here, we propose an enzyme-free strategy for the detection of phosphorothiolate pesticides, a major class of OPPs with P-S bonds, by using hyaluronan-tyrosine-gold nanoparticles (HA-Tyr-AuNPs) as the colorimetric probe. The strategy is based on the concept that the surface of AuNPs can be directly modified by the hydrolyzate of the phosphorothiolate in a strongly basic HA-Tyr-AuNP colloidal solution, which in turn affects the aggregation state of AuNPs and hence produces a red-to-blue color change. This method is very simple in design and easy to operate. The detection requires only the addition of the analyte into the HA-Tyr-AuNP colloidal solution at room temperature. By virtue of the absorbance variations of the analytical system, the limit of detection for phorate is as low as 0.005 mu g mL(-1) (i.e., 0.006 mg kg(-1)), which is lower than the maximum residue limit (MRL) allowed by the European Union. More importantly, phorate residues in real samples can be directly detected by this method.
外文关键词：LIQUID-LIQUID MICROEXTRACTION; SURFACE-PLASMON RESONANCE; FLAME PHOTOMETRIC DETECTION; DUAL-READOUT ASSAY; ORGANOPHOSPHORUS PESTICIDES; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; SILVER NANOPARTICLES; SENSITIVE DETECTION; RESIDUES
作者：Yang, Limin; Zhang, Xiaohui; Li, Huiping; Jiang, Lei
作者单位：China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Shandong, Peoples R China..
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.9
出版年份：2017
出版刊次：11
点击下载：基于透明质酸酪氨酸金纳米粒子的无酶比色探针检测硫代磷酸酯农药残留