中文摘要：食品中病原菌的存在已经造成了日益严重的经济和公共卫生问题，迫切需要能够迅速检测出病原菌的新技术，传统的食品安全评估方法虽然可靠，但是我们需要灵敏度更高、具有可选择性、速度更快且成本效益更低的检测方法；本文综述了可提供有效食品细菌检测的生物识别转导技术，讨论了各项技术的优缺点，并介绍了目前正在开发的新兴生物或纳米传感器技术，总结了食品病原菌监测所面临的机遇和挑战，并着重讨论了最流行的生物识别和纳米传导机制的优缺点。
 
外文摘要：The presence of unsafe levels of microorganisms in food constitutes a growing economic and public health problem that necessitates new technology for their rapid detection along the food continuum from production to consumption. While traditional techniques are reliable, there is a need for more sensitive, selective, rapid, and cost-effective approaches for food safety evaluation. Methods such as microbiological counts are sufficiently accurate and inexpensive, and are capable of determining presence and viability for most pathogens. However, these techniques are time consuming, involve destructive sampling, and require trained personnel and biosafety-certified facilities for analysis. Molecular techniques such as the polymerase chain reaction have greatly improved analytical capability over the last decade, achieving shorter analysis time with quantitative data and strain specificity, and in some cases the ability to discriminate cell viability. The emerging field of nanosensors/biosensors has demonstrated a variety of devices that hold promise to bridge the gap between traditional plate counting and molecular techniques. Many nanosensors/biosensors are rapid, portable, accurate devices that can be used as an additional screening tool for identifying unsafe levels of microorganisms in food products with no need for pre-enrichment. In this review, we provide a brief overview of available biorecognition-transduction techniques for detecting bacteria in food. We then discuss the advantages and disadvantages of each technique, and describe some recent biosensor or nanosensor technologies that are under development. We conclude by summarizing the opportunities and challenges in the field of pathogen monitoring in food systems and we focus the discussion on the strengths/weaknesses of the most popular biorecognition agents and transducer nanomaterials for biosensing.
外文关键词：biosensor, nanotechnology, pathogen, rapid monitoring, spoilage
作者：Vanegas, Diana C.; Gomes, Carmen L.; Cavallaro, Nicholas D.;Giraldo-Escobar, Daniel; McLamore, Eric S.
作者单位：Univ Florida, Agr & Biol Engn, 1741 Museum Rd, Gainesville, FL 32606 USA.
期刊名称：COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
期刊影响因子：5.974
出版年份：2017
出版刊次：11
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