中文摘要：马铃薯是以色列产量最大的农作物，而马铃薯Y病毒是对马铃薯造成严重损害的病毒，人们通常使用发芽的块茎通过酶联免疫吸附试验来检测病毒，但是需要至少六周才能得出结果，因此迫切需要一个快速检测的方法，如TaqMan实时荧光定量PCR检测方法，可直接对收获的块茎进行病毒感染检测，本文还对上述两种检测方法进行了比较，收集2013-2015年三年春季收获的14种不同品种的马铃薯块茎，两种方法检测结果的一致性并没有统计学意义，但是TaqMan实时荧光定量PCR检测方法能够更好地区分块茎是否适合种植，马铃薯Y病毒感染率低于5%的适合种植，否则不适合种植；因此，TaqMan实时荧光定量PCR方法适用于批量种植马铃薯中的马铃薯Y病毒检测。
外文摘要：Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the "Growing-On test"), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013-2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate (< 5% by Growing On test; suitable for seed) and those unsuitable for seed (>= 5% by Growing On test). Therefore, qPCR is an appropriate technique for determination of the PVY infection rate of seed tuber lots in Israel.
外文关键词：Potato virus Y, PVY, Seed potatoes, Real Time PCR, Growing-On test, Virus detection
作者：Avrahami-Moyal, Liat; Tam, Yehudit; Brumin, Marina; Prakash, Shanmugam;Leibman, Diana; Pearlsman, Malenia; Bornstein, Menachem; Sela, Noa; Zeidan, Mouhammad; Dar, Zion; Zig, Uri; Gal-On, Amit; Gaba, Victor
作者单位：ARO Volcani Ctr, Dept Plant Pathol & Weed Sci, HaMaccabim Rd,POB 15159, IL-7528809 Rishon Letsiyon, Israel
期刊名称：PHYTOPARASITICA
期刊影响因子：0.882
出版年份：2017
出版刊次：9
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