中文摘要：miRNA的转基因表达是农业生物技术领域植物性食物增加的普遍方法。新兴研究包括两类，一类强调了转基因miRNA作为新型膳食疗法的可能性，一类提出了潜在食品安全问题，如有害的miRNA被吸收并具有生物活性。因此有必要对转基因作物中转基因miRNA的生物利用度进行评估。研究发现，植物的消化稳定性和miRNA的高度表达并不等同于生物利用度。研究结果表明，在使用转基因食品作为平台时，应采用新的工程策略以提高miRNA的生物利用度。
外文摘要：Background: Transgenic expression of small RNAs is a prevalent approach in agrobiotechnology for the global enhancement of plant foods. Meanwhile, emerging studies have, on the one hand, emphasized the potential of transgenic microRNAs (miRNAs) as novel dietary therapeutics and, on the other, suggested potential food safety issues if harmful miRNAs are absorbed and bioactive. For these reasons, it is necessary to evaluate the bioavailability of transgenic miRNAs in genetically modified crops. Results: As a pilot study, two transgenic Arabidopsis lines ectopically expressing unique miRNAs were compared and contrasted with the plant bioavailable small RNA MIR2911 for digestive stability and serum bioavailability. The expression levels of these transgenic miRNAs in Arabidopsis were found to be comparable to that of MIR2911 in fresh tissues. Assays of digestive stability in vitro and in vivo suggested the transgenic miRNAs and MIR2911 had comparable resistance to degradation. Healthy mice consuming diets rich in Arabidopsis lines expressing these miRNAs displayed MIR2911 in the bloodstream but no detectable levels of the transgenic miRNAs. Conclusions: These preliminary results imply digestive stability and high expression levels of miRNAs in plants do not readily equate to bioavailability. This initial work suggests novel engineering strategies be employed to enhance miRNA bioavailability when attempting to use transgenic foods as a delivery platform.
外文关键词：Dietary microRNAs; Genetically modified organisms; Bioavailability; Digestive stability; MIR2911; Mice
作者：Yang, Jian; Primo, Cecilia; Elbaz-Younes, Ismail; 等.
作者单位：Baylor Coll Med
期刊名称：GENES AND NUTRITION
期刊影响因子：0.0
出版年份：2017
出版刊次：7
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