中文摘要：烟草属于茄科烟草属。它已受到不同研究领域（如生物学、环境化学等）的广泛研究。本文用野生型烟草和转基因烟草的大鼠糖皮质激素受体（GR）基因或发根农杆菌rolC基因，来评价化学物质和恶劣的自然条件（干燥、热应力）对其无机成分的影响。作者选择了4个标记物，分别是Na、K、CI-和NO3-。另外，用原子发射光谱对测定阳离子；用离子色谱法和电位滴定法测定阴离子：并根据结果比较了这两种技术的潜力。本文确定了阳离子总含量，并研究了转基因对植物胁迫刺激能力的影响：GR基因的引入似乎大大减少了无机污染物的胁迫和吸附作用。rolC可使植物对Cr的在吸收能力提高，同时降低其释放能力：这种操作可用于植物修复。
外文摘要：Nicotiana is a well characterized genus of Solanaceae. It is widely studied in literature in different research fields, such as biology, environmental chemistry, etc. In this study Nicotiana langsdolffii wilde type plants and transgenic for the rat glucocorticoid receptor gene (GR) or Agrobacterium rhizogenes roIC gene were used to value the effect of exposure to chemical compounds (Cr) and to harsh physical conditions (dryness, heat stress) on the inorganic compositions in plants. Four markers were selected, namely Na, K, CI- and NO3-. Different procedures of extraction were tested: water permitted to obtain the best conditions for the determination of four selected markers. The analysis was carried out by atomic emission spectroscopy for the determination of the cations; the anions were determined both by ion chromatography and potentiometry: the results obtained by these two techniques and their potentiality were compared. The total cation content (Al, Ca, Cr, Fe, K, Mg, Mn, Na, P, Si and Zn) was also determined. The effect of the genetic modifications on the capacity of the plant to contrast the stress stimuli was valued: the introduction of GR gene seems to greatly reduce the effect caused by the stresses and the adsorption of the inorganic pollutants. The modification with rolC increases the absorption capacity of the plant toward Cr and at the same time reduces its release: this behavior can be exploited in phytoremediation applications. 
外文关键词：Nicotiana transgenic plants; Rat glucocorticoid receptor roIC; Inorganic stress markers; Chemometrics
作者：Malandrino, Mery; Giacomino, Agnese; Karthik, Mani; 等
作者单位：Univ Turin
期刊名称：MICROCHEMICAL JOURNAL
期刊影响因子：3.034
出版年份：2017
出版刊次：9
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