中文摘要：最近的多项研究均强调了气候变化所引起的植物转基因沉默现象，为了了解不同氮素水平下CO2浓度升高对转基因水稻中外源BT毒素及转基因表达的影响，本文在氮素水平分别为1/8、1/4、1/2、1和2的情况下，观察两个级别的CO2浓度下BT转基因水稻中的生物量、外源BT毒素、BT转基因表达及甲基化状态，研究结果表明，大气CO2浓度的升高将触发BT转基因水稻中BT毒蛋白表达的上调，特别是在适当增加氮素供应的情况下；在某种程度上，尽管植物组织总蛋白减少且植物生长受到限制，氮素供应在1/4和1/2水平时，外源bt转基因表达会降低；但对大多数转基因植物来说，转基因沉默和表观遗传上的改变仍然是不可预测的随机事件，而对转基因水稻来说，氮素供应可以促进转bt基因水稻bt毒素的表达，特别是在CO2浓度升高的情况下。
外文摘要：Recent studies have highlighted great challenges of transgene silencing for transgenic plants facing climate change. In order to understand the impacts of elevated CO2 on exogenous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different levels of N-fertilizer supply, we investigated the biomass, exogenous Bt toxins, Bt-transgene expression and methylation status in Bt rice exposed to two levels of CO2 concentrations and nitrogen (N) supply (1/8, 1/4, 1/2,1 and 2 N). It is elucidated that the increased levels of global atmospheric CO2 concentration will trigger up-regulation of Bt toxin expression in transgenic rice, especially with appropriate increase of N fertilizer supply, while, to some extent, the exogenous Bt-transgene expression is reduced at sub-N levels (1/4 and 1/2N), even though the total protein of plant tissues is reduced and the plant growth is restricted. The unpredictable and stochastic occurrence of transgene silencing and epigenetic alternations remains unresolved for most transgenic plants. It is expected that N fertilization supply may promote the expression of transgenic Bt toxin in transgenic Bt rice, particularly under elevated CO2.
外文关键词：HELIOTHIS-VIRESCENS LEPIDOPTERA; BACILLUS-THURINGIENSIS; INSECT RESISTANCE; CRY1AC; NOCTUIDAE; PROTEINS; QUANTIFICATION; SUSCEPTIBILITY; EXPRESSION; BOLLWORM
作者：Little, Nathan S.; Mullen, R. Michelle; Allen, K. Clint; Tyler, Heather L.
作者单位：USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA.
期刊名称：SOUTHWESTERN ENTOMOLOGIST
期刊影响因子：0.482
出版年份：2017
出版刊次：12
点击下载：不同氮素水平下CO2浓度升高对转基因水稻中外源BT毒素及转基因表达的影响