中文摘要：草地贪夜蛾是Cry2Ab2转基因玉米和棉花最主要的害虫，本研究首次对来自抗草地贪夜蛾Cry2Ab2基因玉米的交叉抗性进行了评估，浓度响应的生物活性测定结果表明：相对于敏感Cry2Ab2转基因玉米，抗性Cry2Ab2转基因玉米与Cry1F、Cry1A.105、Cry2Ab2、Cry2Ae以及Vip3A 的抗性比率分别为是-1.4、1.2、>26.7、>10.0以及-1.1；总之，抗草地贪夜蛾Cry2Ab2基因玉米与Cry1F、Cry1A.105、Vip3A等蛋白以及转基因BT玉米和棉花没有交叉抗性，但是与Cry2Ae蛋白和表达相似BT蛋白的转基因BT作物有显著的交叉抗性。
外文摘要：BACKGROUND Gene-pyramiding by combining two or more dissimilar Bacillus thuringiensis (Bt) proteins into a crop has been used to delay insect resistance. The durability of gene-pyramiding can be reduced by cross-resistance. Fall armyworm, Spodoptera frugiperda, is a major target pest of the Cry2Ab2 protein used in pyramided Bt corn and cotton. Here, we provide the first experimental evaluation of cross-resistance in S. frugiperda selected with Cry2Ab2 corn to multiple Bt sources including purified Bt proteins, Bt corn and Bt cotton. RESULTSConcentration-response bioassays showed that resistance ratios for Cry2Ab2-resistant (RR) relative to Cry2Ab2-susceptible (SS) S. frugiperda were -1.4 for Cry1F, 1.2 for Cry1A.105, >26.7 for Cry2Ab2, >10.0 for Cry2Ae and -1.1 for Vip3A. Larvae of Cry2Ab2-heterozygous (RS), SS and RR S. frugiperda were all susceptible to Bt corn and Bt cotton containing Cry1 (Cry1F or Cry1A.105) and/or Vip3A proteins. Pyramided Bt cotton containing Cry1Ac+Cry2Ab2 or Cry1Ab+Cry2Ae were also effective against SS and RS, but not RR. CONCLUSIONThese findings suggest that Cry2Ab2-corn-selected S. frugiperda is not cross-resistant to Cry1F, Cry1A.105 or Vip3A protein, or corn and cotton plants containing these Bt proteins, but it can cause strong cross-resistance to Cry2Ae and Bt crops expressing similar Bt proteins. (c) 2017 Society of Chemical Industry.
 
外文关键词：Bacillus thuringiensis; cross-resistance; Bt corn; Bt cotton; Spodoptera frugiperda; resistance, Cry2Ab
作者：Fei Yang;David L Kerns;Graham P Head;Paula Pricea and Fangneng Huang;
作者单位：Louisiana State University Agricultural Center
期刊名称：PEST MANAGEMENT SCIENCE
期刊影响因子：3.253
出版年份：2017
出版刊次：12
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