中文摘要：棉铃虫是一种全球最具破坏性的棉花害虫，我国主要依靠1997年研制的可产生单一BT毒蛋白的转基因棉花来防治棉铃虫，在我国北方发现了田间棉铃虫具有Cry1Ac抗性，Vip3Aa与Cry1Ac蛋白有不同的结构和行为模式，
本研究对2014年中国棉花产区棉铃虫对BT vip3Aa毒素的敏感基线进行了评估，12个地区田间种群的LC50值在0.05-1.311μg/cm2之间，是自然种群的25倍；说明四株高抗Cry1Ac或Cry2Ab棉铃虫菌株对Vip3Aa没有交叉抗性，本文所获敏感基线数据可作为检测田间棉铃虫Vip3Aa抗性的比较基准。
 
外文摘要：The cotton bollworm Helicoverpa armigera (Hubner) is one of the most damaging cotton pests worldwide. In China, control of this pest has been dependent on transgenic cotton producing a single Bacillus thuringiensis (Bt) protein Cry1Ac since 1997. A small, but significant, increase in H. armigera resistance to Cry1Ac was detected in field populations from Northern China. Since Vip3Aa has a different structure and mode of action than Cry proteins, Bt cotton pyramids containing Vip3Aa are considered as ideal successors of Cry1Ac cotton in China. In this study, baseline susceptibility of H. armigera to Vip3Aa was evaluated in geographic field populations collected in 2014 from major cotton-producing areas of China. The LC50 values of 12 field populations ranged from 0.05 to 1.311 mu g/cm(2), representing a 25-fold range of natural variation among populations. It is also demonstrated that four laboratory strains of H. armigera with high levels of resistance to Cry1Ac or Cry2Ab have no cross-resistance to Vip3Aa protein. The baseline susceptibility data established here will serve as a comparative reference for detection of field-evolved resistance to Vip3Aa in H. armigera after future deployment of Bt cotton pyramids in China.
 
外文关键词：Helicoverpa armigera, Vip3Aa, Cry1Ac, Cry2Ab, susceptibility, cross-resistance
作者：Wei, Yiyun; Wu, Shuwen; Yang, Yihua; Wu, Yidong
作者单位：Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China.
期刊名称：TOXINS
期刊影响因子：3.03
出版年份：2017
出版刊次：4
点击下载：田间棉铃虫对BT vip3Aa毒素的敏感基线及vip3Aa和Cry毒素的交叉抗性缺陷研究