中文摘要：CRY1毒素的检测主要依赖于基于特异性单克隆抗体（mAb）的免疫测定法，本研究采用七种CRY1毒素混合免疫遗传算法制备特异性单克隆抗体，结果显示，其中五种单克隆抗体具有不同的特性，尤其是5-E8，可以识别七种CRY1毒素，本文基于5-e8单克隆抗体，采用双抗夹心酶联免疫吸附法（ELISA）检测对Cry2A和vip3没有交叉抗性的七种Cry1毒素，检测限和定量限分别为6.37-11.35 ng/mL和17.36-24.48 ng/mL，回收率在78% -110%之间，双抗夹心酶联免疫吸附法在农产品中CRY1毒素监测方面非常有用，混合免疫遗传算法为在单个免疫周期内同时生产多种单克隆抗体开辟了新的道路。
外文摘要：The detections of Cry1 toxins are mainly dependent on immunoassays based on specific monoclonal antibodies (mAb). In the present study, a mixture immunization with seven Cry1 toxins was administered. The results showed that five mAbs with different characteristics, especially one mAb named 5-E8 which could recognize all the seven Cry1 toxins were obtained. Based on the 5-E8 mAb, a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) which can specifically detect the seven Cry1 toxins without cross-reactivity to Cry2A and vip3 was developed with the limit of detection (LOD) and limit of quantification (LOQ) of 6.37-11.35 ng mL(-1) and 17.36-24.48 ng mL(-1), respectively. The recovery tests showed that the recoveries ranged from 78% to 110% within the quantitation range (LOQ-100 ng mL(-1)). The established DAS-ELISA can be a useful tool for monitoring the Cry1 toxins in agricultural products. Mixture immunization opens a new path for producing diverse mAbs simultaneously in a single immunization circle. (C) 2017 Elsevier Inc. All rights reserved.
外文关键词：Mixture immunization, Cry1 family, Diverse hybridomas, Monoclonal antibody, DAS-ELISA
作者：Dong, Sa; Zhang, Cunzheng; Liu, Yuan; Zhang, Xiao; Xie, Yajing; Zhong, Jianfeng; Xu, Chongxin; Liu, Xianjin
作者单位：Food Qual Safety & Detect Res Jiangsu Prov, Nanjing 210014, Jiangsu, Peoples R China.
期刊名称：ANALYTICAL BIOCHEMISTRY
期刊影响因子：2.334
出版年份：2017
出版刊次：8
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