中文摘要：亚洲玉米螟已经对Cry1Ie室内汰选49代品系的抗性提高了800倍，本文通过新生儿暴露试验测定了亚洲玉米螟对Cry1Ie的抗性遗传模式以及对其他BT毒素的交叉抗性。回交生物量测定表明，抗性为常染色体遗传，受多个点位控制；抗性菌株对Cry1Ab (0.8倍), Cry1Ac (0.8倍), Cry1F (0.9倍), and Cry1Ah (1.0倍)没有表现出交叉抗性。本研究不仅描述了抗性表现，还建议在转BT基因玉米发展中Cry1Ie蛋白与Cry1Ab, Cry1Ac, Cry1F和 Cry1Ah交叉表达的适用性。
外文摘要：A strain of the Asian corn borer (ACB), Ostrinia furnacalis (Guenee), has evolved >800-fold resistance to Cry1Ie (ACB-IeR) after 49 generations of selection. The inheritance pattern of resistance to Cry1Ie in ACB-IeR strain and its cross-resistance to other Bt toxins were determined through bioassay by exposing neonates from genetic-crosses to toxins incorporated into the diet. The response of progenies from reciprocal F-1 crosses were similar (LC(50)s: 76.07 vs. 74.32 mu g/g), which suggested the resistance was autosomal. The effective dominance (h) decreased as concentration of Cry1Ie increased. h was nearly recessive or incompletely recessive on Cry1Ie maize leaf tissue (h = 0.02), but nearly dominant or incompletely dominant (h = 0.98) on Cry1Ie maize silk. Bioassay of the backcross suggested that the resistance was controlled by more than one locus. In addition, the resistant strain did not perform cross-resistance to Cry1Ab (0.8-fold), Cry1Ac (0.8-fold), Cry1F (0.9-fold), and Cry1Ah (1.0-fold). The present study not only offers the manifestation for resistance management, but also recommends that Cry1Ie will be an appropriate candidate for expression with Cry1Ab, Cry1Ac, Cry1F, or Cry1Ah for the development of Bt maize.
 
外文关键词：Ostrinia furnacalis, Bacillus thuringiensis, inheritance, cross-resistance, resistance management
作者：Wang, Yueqin; Yang, Jing; Quan, Yudong; Wang, Zhenying; Cai, Wanzhi; He,Kanglai
作者单位：Department of Entomology, China Agricultural University, Beijing 100193, China.
期刊名称：TOXINS
期刊影响因子：3.03
出版年份：2017
出版刊次：8
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