中文摘要：西部玉米根虫是北美洲最严重的玉米害虫之一，防治该害虫的其中一种策略是使用可产生一种或多种晶状毒素的转基因BT玉米，为了延缓害虫对BT毒素的抗性，将非转基因玉米与转基因BT玉米结合种植，而影响这种抗性延缓策略的两个因素就是隐性抗性遗传和适合度代价，本文对两个抗转基因Cry3Bb1玉米的西部玉米根虫的遗传规律和适合度代价进行了评估，结果显示：抗性为非隐性遗传，温室试验中未检测到抗性的适合度代价，实验室试验中确定了延缓幼虫发育的适合度代价，但是并没有其他适合度代价；这种非隐形抗性遗传和最小的适合度代价发现强调了西部玉米根虫对转基因Cry3Bb1玉米抗性快速进化的可能性，将有助于改善害虫的抗性管理策略。
外文摘要：The western corn rootworm, Diabrotica virgifera virgifera LeConte, is among the most serious insect pests of maize in North America. One strategy used to manage this pest is transgenic maize that produces one or more crystalline (Cry) toxins derived from the bacterium Bacillus thuringiensis (Bt). To delay Bt resistance by insect pests, refuges of non-Bt maize are grown in conjunction with Bt maize. Two factors influencing the success of the refuge strategy to delay resistance are the inheritance of resistance and fitness costs, with greater delays in resistance expected when inheritance of resistance is recessive and fitness costs are present. We measured inheritance and fitness costs of resistance for two strains of western corn rootworm with field-evolved resistance to Cry3Bb1 maize. Plant-based and diet-based bioassays revealed that the inheritance of resistance was non-recessive. In a greenhouse experiment, in which larvae were reared on whole maize plants in field soil, no fitness costs of resistance were detected. In a laboratory experiment, in which larvae experienced intraspecific and interspecific competition for food, a fitness cost of delayed larval development was identified, however, no other fitness costs were found. These findings of non-recessive inheritance of resistance and minimal fitness costs, highlight the potential for the rapid evolution of resistance to Cry3Bb1 maize by western corn rootworm, and may help to improve resistance management strategies for this pest.
外文关键词：Bacillus thuringiensis, maize, Diabrotica virgifera virgifera, fitness cost, inheritance, resistance management, refuge strategy
作者：Paolino, Aubrey R.; Gassmann, Aaron J
作者单位：Iowa State Univ, Dept Entomol, Ames, IA 50011 USA.
期刊名称：TOXINS
期刊影响因子：3.03
出版年份：2017
出版刊次：5
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