中文摘要：本文开发了一个全因子设计，用于通过超高效液相色谱-串联质谱法检测胡萝卜、玉米、甜瓜、大米、大豆、青贮饲料、烟草、木薯、莴苣、小麦等植物中的72种农药残留。考虑到基质的复杂性和校准的需要，本文提出了一个基于单级样本加标的校准方法，并与基质匹配校准、程序标准校准、稀释加标校准进行比较；所有方法的验证参数都很满意，回收率在70-120%之间，相对标准差小于等于20%。验证结果表明，单级加标校准是一种有效的校准方法且最为实用，检测限为4.8-48μg/kg，定量限为16-160μg/kg；应用该方法检测不同植物中20中农药残留，并采用Z值≤2的校准方法进行量化，QuEChERS-超高效液相色谱-串联质谱法和简单有效的校准程序在不同农作物农药残留检测中获得了较满意的结果，是常规检测分析的一个很好选择。
 
外文摘要：full factorial design, was developed for the determination of 72 pesticides in plant parts of carrot, corn, melon, rice, soy, silage, tobacco, cassava, lettuce and wheat by ultra-high-performance liquid chromatographic tandem mass spectrometry (UHPLC-MS/MS). Considering the complexity of these matrices and the need of use calibration in matrix, a new calibration approach based on single level standard addition in the sample (SLSAS) was proposed in this work and compared with the matrix-matched calibration (MMC), the procedural standard calibration (PSC) and the diluted standard addition calibration (DSAC). All approaches presented satisfactory validation parameters with recoveries from 70 to 120% and relative standard deviations <= 20%. SLSAS was the most practical from the evaluated approaches and proved to be an effective way of calibration. Method limit of detection were between 4.8 and 48 mu g kg(-1) and limit of quantification were from 16 to 160 mu g kg(-4). Method application to different kinds of plants found residues of 20 pesticides that were quantified with z-scores values <= 2 in comparison with other calibration approaches. The proposed QuEChERS method combined with UHPLC-MS/MS analysis and using an easy and effective calibration procedure presented satisfactory results for pesticide residues determination in different crop plants and is a good alternative for routine analysis. (C) 2017 Elsevier B.V. All rights reserved.
外文关键词：Pesticides, Plants, Standard addition, QuEChERS, UHPLC-MS/MS
作者：Viera, Mariela S.; Rizzetti, Tiele M.; de Souza, Maiara P.; Martins, Manoel L.; Prestes, Osmar D.; Adaime, Martha B.; Zanella, Renato
作者单位：Univ Fed Santa Maria, Chem Dept, Lab Pesticide Residues Anal LARP, BR-97105900 Santa Maria, RS, Brazil.
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.981
出版年份：2017
出版刊次：12
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