中文摘要：脱氧雪腐镰刀菌烯醇、T2毒素和赭曲霉毒素A是玉米和小麦中最常见的三种真菌毒素，色谱和质谱仪是最常用的真菌检测分析方法，但这些方法耗时耗力、操作困难且成本较高，而免疫胶体金法是一种适合现场测试的快速检测方法，但在同时检测真菌毒素时面临着检测条件优化和样本制备程序困难的难题，因此，本文采用三种不同涂层、不同浓度的三条测试线(T-1, T-2, T-3)来分别检测脱氧雪腐镰刀菌烯醇、T2毒素和赭曲霉毒素A毒素；T-1, T-2, T-3三条测试线分别在浓度达到1 mg/kg、1 mg/kg、5 μg/kg时消失。本文还开发了一种采用固相分散萃取和胶体金试纸条清洗的样本制备方法，使得小麦中毒素检测更加容易且能够满足中国官方规定的残留限制，该方法可明显改善人们在农产品加工和采购中的食品安全和身体健康。
外文摘要：Deoxynivalenol (DON, also called as Vomitoxin), T2 toxin and Ochratoxin A (OTA) are three fungal toxins commonly found in corn or wheat. Chromatography and Mass spectrometer are main analysis methods for testing these mycotoxins. But these methods are time consuming, difficult to operate, and high cost on human resources and economy. Immune Colloidal Gold Assay (ICGA) is a rapid testing method suitable for on-spot testing. Unfortunately, the main reason for ICGA difficult to test these three toxins simultaneously is the optimization of the testing condition and sample preparation procedures. In this study, by utilizing three different coating antigens with different concentration, three test lines were set (T-1, T-2, T-3), for detecting DON, T2 toxin and OTA, respectively. The Test Line disappears when the concentration reach 1 mg/kg (for DON), 1 mg/kg (for T2) and 5 mu g/kg (for OTA), separately. We also developed a sample preparation method by using MSPD for extraction and cleaning of the three toxins above for the simultaneously ICGA. That makes it easier for detecting toxins in wheat and for assurance of the food that can meet official limit of residue restrictions of China. The method could obviously improve the people's food safety and human health in real practice of agro-products' processing and purchasing. (C) 2017 Friends Science Publishers
外文关键词：Deoxynivalenol, T2 toxin, Ochratoxin A, Colloidal gold, Immunoassay, Matrix solid-phase dispersion cleaning
作者：Jin, Nuo; Wang, Miao; Fan, Bei; He, Yan; Huang, Yatao; Wang, Fengzhong;Liu, Yang
作者单位：Chinese Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Beijing, Peoples R China.
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
期刊影响因子：0.746
出版年份：2017
出版刊次：19
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