中文摘要：蛋白质组学已成为研究转基因作物非预期效果的有效技术。本文利用2-DE和iTRAQ技术对转植酸酶基因玉米（PT）和非转基因玉米（NT）种子进行了蛋白质组学比较分析。本研究在转植酸酶基因玉米（PT）中共检测出了148个差异表达的蛋白（DEPS），包括106个下调和42个上调蛋白。研究结果表明，在转植酸酶基因玉米种子和非转基因玉米种子的蛋白质组中检测到很多差异蛋白，但这些蛋白的差异不是很大；蛋白功能分类表明很多蛋白参与转录修饰、某些核糖体蛋白和热休克蛋白过程，这可能产生对外源基因插入反应的适应性效应。蛋白质相互作用分析表明，所检测的相互作用蛋白主要是核糖体蛋白和热休克蛋白。本文的数据对玉米种子蛋白质组学非预期效果分析提供了新的信息。
外文摘要：Proteomics has become a powerful technique for investigating unintended effects in genetically modified crops. In this study, we performed a comparative proteomics of the seeds of phytase-transgenic (PT) and non-transgenic (NT) maize using 2-DE and iTRAQ techniques. A total of 148 differentially expressed proteins (DEPs), including 106 down-regulated and 42 up-regulated proteins in PT, were identified. Of these proteins, 32 were identified through 2-DE and 116 were generated by iTRAQ. It is noteworthy that only three proteins could be detected via both iTRAQ and 2-DE, and most of the identified DEPs were not newly produced proteins but proteins with altered abundance. These results indicated that many DEPs could be detected in the proteome of PT maize seeds and the corresponding wild type after overexpression of the target gene, but the changes in these proteins were not substantial. Functional classification revealed many DEPs involved in posttranscriptional modifications and some ribosomal proteins and heat-shock proteins that may generate adaptive effects in response to the insertion of exogenous genes. Protein-protein interaction analysis demonstrated that the detected interacting proteins were mainly ribosomal proteins and heat-shock proteins. Our data provided new information on such unintended effects through a proteomic analysis of maize seeds.
作者：Tan, Yanhua; Tong, Zheng; Yang, Qian; 等.
作者单位：Chinese Acad Trop Agr Sci
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2017
出版刊次：8
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