中文摘要：马铃薯块茎的形成是一个复杂的生物学过程，其是由许多因素包括光敏色素、激素、转录因子、RNAs、microRNA等调控。本文研究了StCYP707A1基因对转基因马铃薯块茎形成的作用。作者从Dsire马铃薯中克隆了一个CYP707A1基因，并利用农杆菌介导转化法构建了超表达和反义转基因马铃薯的StCYP707A1。通过PCR分析共鉴定出四个超表达转基因株系和九个反义转基因株系。半定量RT-PCR和荧光定量PCR分析表明，相对于野生型马铃薯，StCYP707A1的转录水平在超表达转基因株系较高，在反义转基因株系中较低。本文进一步分析了转基因和野生马铃薯的块茎形成，研究结果表明，StCYP707A1会通过ABA调控赤霉素浓度进而抑制影响马铃薯块茎形成。
外文摘要：Tuber formation of potato is a complex biological process and is regulated by many factors including phytochrome, hormones, transcription factors, RNAs, microRNAs, etc. In our previous study, CYP707A1, an ABA 8'-hydroxylase gene, was down-regulated in the StCOL antisense transgenic potato stolons, but its relation with potato tuberization has not been studied yet. In this study, to investigate the role of this gene in potato tuberization, we cloned a CYP707A1 gene from potato cultivar D,sir,e and constructed the StCYP707A1 over-expression and anti-sense potato lines by Agrobacterium-mediated transformation. In total, four over-expression transgenic lines and nine anti-sense transgenic lines were confirmed by PCR analysis. Semi-quantitative RT-PCR and qRT-PCR analysis showed that the level of StCYP707A1 transcripts was significantly higher in the over-expression lines (A7 and A9) and lower in the anti-sense lines (F2 and F8), compared to the wild-type control. We further analyzed tuber formation in the transgenic lines and the wild-type control. The result showed that tuber yield per plant and average tuber weight were decreased in A7 and A9 and increased in F2. We also measured the content of ABA and GA(3) in transgenic lines. ABA level was reduced in A7 and A9 and increased in F2. Contrariwise, the concentration of GA(3) was higher in A7 and A9, and lower in F2 than in wild-type control. These results indicate that StCYP707A1 negatively affects potato tuberization through ABA regulation on gibberellic acid concentration.
外文关键词：Potato; StCYP707A1; Tuberization; Regulation; ABA
作者：Liu, Lu; Zhang, Rui-Jie; Zhu, Wen-Jiao; 等.
作者单位：Nanjing Agr Univ
期刊名称：PLANT BIOTECHNOLOGY REPORTS
期刊影响因子：1.422
出版年份：2017
出版刊次：8
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